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Cryo-EM and ED with a Cold-Field Emission Beam and Energy Filtration

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  1. Article

    Open Access

    Author Correction: Structural resolution of a small organic molecule by serial X-ray free-electron laser and electron crystallography

    Kiyofumi Takaba, Saori Maki-Yonekura, Ichiro Inoue, Kensuke Tono in Nature Chemistry (2023)

  2. Article

    Open Access

    Enantioselectivity of discretized helical supramolecule consisting of achiral cobalt phthalocyanines via chiral-induced spin selectivity effect

    Enantioselectivity of helical aggregation is conventionally directed either by its homochiral ingredients or by introduction of chiral catalysis. The fundamental question, then, is whether helical aggregation ...

    Hiroki Aizawa, Takuro Sato, Saori Maki-Yonekura, Koji Yonekura in Nature Communications (2023)

  3. Article

    Open Access

    Measurement of charges and chemical bonding in a cryo-EM structure

    Hydrogen bonding, bond polarity, and charges in protein molecules play critical roles in the stabilization of protein structures, as well as affecting their functions such as enzymatic catalysis, electron tran...

    Saori Maki-Yonekura, Keisuke Kawakami, Kiyofumi Takaba in Communications Chemistry (2023)

  4. Article

    Open Access

    Structural resolution of a small organic molecule by serial X-ray free-electron laser and electron crystallography

    Structure analysis of small crystals is important in areas ranging from synthetic organic chemistry to pharmaceutical and material sciences, as many compounds do not yield large crystals. Here we present the d...

    Kiyofumi Takaba, Saori Maki-Yonekura, Ichiro Inoue, Kensuke Tono in Nature Chemistry (2023)

  5. Article

    Open Access

    Structure of a monomeric photosystem I core associated with iron-stress-induced-A proteins from Anabaena sp. PCC 7120

    Iron-stress-induced-A proteins (IsiAs) are expressed in cyanobacteria under iron-deficient conditions. The cyanobacterium Anabaena sp. PCC 7120 has four isiA genes; however, their binding property and functional ...

    Ryo Nagao, Koji Kato, Tasuku Hamaguchi, Yoshifumi Ueno in Nature Communications (2023)

  6. Article

    Open Access

    Core and rod structures of a thermophilic cyanobacterial light-harvesting phycobilisome

    Cyanobacteria, glaucophytes, and rhodophytes utilize giant, light-harvesting phycobilisomes (PBSs) for capturing solar energy and conveying it to photosynthetic reaction centers. PBSs are compositionally and s...

    Keisuke Kawakami, Tasuku Hamaguchi, Yuu Hirose, Daisuke Kosumi in Nature Communications (2022)

  7. Article

    Open Access

    Machine learning-based real-time object locator/evaluator for cryo-EM data collection

    In cryo-electron microscopy (cryo-EM) data collection, locating a target object is error-prone. Here, we present a machine learning-based approach with a real-time object locator named yoneoLocr using YOLO, a ...

    Koji Yonekura, Saori Maki-Yonekura, Hisashi Naitow in Communications Biology (2021)

  8. Article

    Open Access

    Structure of the far-red light utilizing photosystem I of Acaryochloris marina

    Acaryochloris marina is one of the cyanobacterial species that can use far-red light to drive photochemical reactions for oxygenic photosynthesis. Here, we report the structure of A. marina photosystem I (PSI) re...

    Tasuku Hamaguchi, Keisuke Kawakami, Kyoko Shinzawa-Itoh in Nature Communications (2021)

  9. Article

    Open Access

    High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams

    Photosystem II (PSII) plays a key role in water-splitting and oxygen evolution. X-ray crystallography has revealed its atomic structure and some intermediate structures. However, these structures are in the cr...

    Koji Kato, Naoyuki Miyazaki, Tasuku Hamaguchi, Yoshiki Nakajima in Communications Biology (2021)

  10. Article

    Open Access

    Apple latent spherical virus structure with stable capsid frame supports quasi-stable protrusions expediting genome release

    Picorna-like plant viruses are non-enveloped RNA spherical viruses of ~30 nm. Part of the survival of these viruses depends on their capsid being stable enough to harbour the viral genome and yet malleable eno...

    Hisashi Naitow, Tasuku Hamaguchi, Saori Maki-Yonekura in Communications Biology (2020)