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Book
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Chapter
Cell Fusion-Mediated Nuclear Reprogramming of Somatic Cells
Epigenetic reprogramming of nuclei from differentiated to pluripotent state can be induced by three experimental approaches: nuclear transfer, cell fusion, and transduction of transcription factors. In cell fu...
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Protocol
Generation of Induced Pluripotent Stem Cells from Human Amnion Cells
Induced pluripotent stem (iPS) cells have been generated through nuclear reprogramming of somatic cells via retrovirus- or lentivirus-mediated transduction of exogenous reprogramming factors OCT3/4, SOX2, KLF4, a...
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Chapter
Epilogue
Recent fascinating developments in the interwoven areas of molecular biology, biochemistry, and biomedicine are starting to provide explanations for many complicated “phenomena,” enabling them to be broken dow...
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Article
Familiarity and Prudence of the Japanese Public with Research into Induced Pluripotent Stem Cells, and Their Desire for its Proper Regulation
The lack of knowledge of current public attitudes towards basic research into induced pluripotent stem cells (iPSCs) is a serious problem when considering appropriate ways of governance regarding research and ...
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Article
Target chromosomes of inducible deletion by a Cre/inverted loxP system in mouse embryonic stem cells
Chromosomal deletions are widely involved in serious genetic diseases and in the pathogenesis of cancers. These deletions often generate loss of heterozygosity (LOH) of one of the alleles of a tumor suppressor...
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Protocol
Nuclear Reprogramming
Nuclear reprogramming is an intriguing phenomenon, in which specialized somatic cells reacquire pluripotency through the global resetting of epigenetic modifications without changes occurring to their nuclear ...
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Protocol
Nuclear Reprogramming of Somatic Nucleus Hybridized With Embryonic Stem Cells by Electrofusion
Cell fusion is a powerful tool for understanding the molecular mechanisms of epigenetic reprogramming. In hybrid cells of somatic cells and pluripotential stem cells, including embryonic stem (ES) and embryoni...
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Protocol
Epigenetic Reprogramming of Somatic Genomes by Electrofusion With Embryonic Stem Cells
Cell fusion is an approach for combining genetic and epigenetic information between two different types of cells. Electrofusion for generating hybrid cells between mouse embryonic stem cells and somatic cells,...