91 Result(s)

Article

Crystal Structure of Gallium(III) Complex of 1,4,7-Triazacyclononane-N,N′, N″-triacetate

Akinori Jyo, Takashi Kohno, Yuichi Terazono, Shigeaki Kawano in Analytical Sciences (1990)

Article

Crystal Structure of the Aluminum(III) Complex of 1,4,7-Triazacyclononane-N,N′,N″-triacetate

Akinori Jyo, Takashi Kohno, Yuichi Terazono, Shigeaki Kawano in Analytical Sciences (1990)

Article

Detection of amplified genomic sequences in human small-cell lung carcinoma cells by arbitrarily primed-PCR genomic fingerprinting

The arbitrarily primed-PCR (AP-PCR) genomic fingerprinting method was applied to evaluate its effectiveness in detecting and characterizing amplified DNA fragments in two small-cell lung carcinoma (SCLC) cell...

Tomoko Okazaki, Junko Takita, Takashi Kohno, Hiroshi Handa, J. Yokota in Human Genetics (1996)

Article

Cloning of a human homolog of the yeast OGG1 gene that is involved in the repair of oxidative DNA damage

We report the cloning of a human homolog of the yeast OGG1 gene, which encodes a DNA glycosylase that excises an oxidatively damaged form of guanine, 8-hydroxyguanine (also known as 7,8-dihydro-8-oxoguanine). Sin...

Kiyomitsu Arai, Kazuhiro Morishita, Kazuya Shinmura, Takashi Kohno… in Oncogene (1997)

Protocol

PCR Fingerprinting for Detection of Deleted or Amplified Sequences in Human Cancer

Genomic fingerprinting is one of several methods for screening human genome to identify genetic alterations in cancer cells. Arbitrarily primed polymerase chain reaction (AP-PCR) is a PCR-based genomic fingerp...

Takashi Kohno, Jun Yokota in PCR in Bioanalysis (1998)

Article

Genomic structure and chromosomal localization of the mouse Ogg1 gene that is involved in the repair of 8-hydroxyguanine in DNA damage

8-Hydroxyguanine (7,8-dihydro-8-oxoguanine: oh⁸Gua) is a damaged form of guanine induced by oxygen-free radicals and causes GC to TA transversions. Previously we isolated the hOGG1 gene, a human homolog of the ye...

Masachika Tani, Kazuya Shinmura, Takashi Kohno, Toshihiko Shiroishi… in Mammalian Genome (1998)

Article

Identification of CpG islands hypermethylated in human lung cancer by the arbitrarily primed-PCR method

DNA hypermethylation is believed to be involved in human carcinogenesis, since it suppresses the transcription of defined genes and is associated with chromosomal instability. In this study, we identified CpG ...

Takashi Kohno, Masashi Kawanishi, Johji Inazawa, J. Yokota in Human Genetics (1998)

Article

Genetic polymorphisms and alternative splicing of the hOGG1 gene, that is involved in the repair of 8-hydroxyguanine in damaged DNA

The hOGG1 gene encodes a DNA glycosylase that excises 8-hydroxyguanine (oh⁸Gua) from damaged DNA. Structural analyses of the hOGG1 gene and its transcripts were performed in normal and lung cancer cells. Due to a...

Takashi Kohno, Kazuya Shinmura, Masahiko Tosaka, Masachika Tani, Su-Ryang Kim… in Oncogene (1998)

Article

Correlation between the status of the p53 gene and survival in patients with stage I non-small cell lung carcinoma

The association of p53 abnormalities with the prognosis of patients with non-small cell lung carcinoma (NSCLC) has been extensively investigated to date, however, this association is still controversial. There...

Yoshio Tomizawa, Takashi Kohno, Takeshi Fujita, Masaharu Kiyama, Ryusei Saito… in Oncogene (1999)

Article

Somatic mutations and genetic polymorphisms of the PPP1R3 gene in patients with several types of cancers

Recently, we found nonsense and missense mutations of the PPP1R3 (protein phosphatase 1, regulatory subunit 3) gene in diverse human cancer cell lines and primary lung carcinomas, indicating that PPP1R3 functions...

Satoshi Takakura, Takashi Kohno, Kimihiro Shimizu, Susumu Ohwada, Aikou Okamoto… in Oncogene (2000)

Article

Identification of a 428-kb homozygously deleted region disrupting the SEZ6L gene at 22q12.1 in a lung cancer cell line

Frequent allelic losses on chromosome 22q in small cell lung carcinomas (SCLCs) and advanced non-small cell lung carcinomas indicate the presence of tumor suppressor gene(s) on this chromosome arm. We detected...

Michiho Nishioka, Takashi Kohno, Mina Takahashi, Toshiro Niki, Tesshi Yamada… in Oncogene (2000)

Article

Genetic alterations responsible for metastatic phenotypes of lung cancer cells

It is now widely accepted that human carcinogenesis is a multi-step process and phenotypic changes during cancer progression reflect the sequential accumulation of genetic alterations in cells. Thus, in order ...

Jun Yokota, Michiho Nishioka, Masachika Tani… in Clinical & Experimental Metastasis (2003)

Article

Molecular processes of chromosome 9p21 deletions in human cancers

Interstitial deletions of the chromosome 9p21 segment encoding the p16/CDKN2A tumor suppressor gene (i.e., 9p21 deletions) are frequently observed in a variety of human cancers. A majority of these deletions in l...

Shigeru Sasaki, Yukiko Kitagawa, Yoshitaka Sekido, John D Minna… in Oncogene (2003)

Article

Identification of genes whose expression is upregulated in lung adenocarcinoma cells in comparison with type II alveolar cells and bronchiolar epithelial cells in vivo

To identify genes whose expression is upregulated in lung adenocarcinoma (AdC) cells in comparison with noncancerous peripheral lung epithelial cells, type II alveolar cells and bronchiolar epithelial cells, a...

Keiko Kobayashi, Michiho Nishioka, Takashi Kohno, Masaki Nakamoto… in Oncogene (2004)

Article

Probing the chromosome 9p21 region susceptible to DNA double-strand breaks in human cells in vivo by restriction enzyme transfer

A restriction enzyme, MspI, was introduced into cultured human cells as a probe to detect genomic regions susceptible to DNA double-strand breaks (DSBs). A 2 h exposure to MspI at a concentration of 8 U/μl produc...

Masanori Sato, Hiroki Sasaki, Teruhisa Kazui, Jun Yokota, Takashi Kohno in Oncogene (2005)

Article

Carvedilol Exerts More Potent Antiadrenergic Effect than Metoprolol in Heart Failure

Background: It is still uncertain whether or not there is a difference between metoprolol and carvedilol for the treatment of congestive heart failure. We attempted to determine the difference between the two β-b...

Takashi Kohno, Tsutomu Yoshikawa, Akihiro Yoshizawa… in Cardiovascular Drugs and Therapy (2005)

Article

Bispectral index monitor in anesthetic management during thyroidectomy of a patient with poorly controlled Graves' disease

Takashi Kohno, Ko Takakura, Maki Mizogami, Kazuyuki Ooshima in Journal of Anesthesia (2006)