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A multi-laboratory benchmark study of isothermal titration calorimetry (ITC) using Ca2+ and Mg2+ binding to EDTA
A small-scale ITC benchmarking study was performed involving 9 biophysics laboratories/facilities, to evaluate inter-laboratory and intra-laboratory basal levels of uncertainty. Our prime goal was to assess a ...
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Article
Aggregation Behavior of pHLIP in Aqueous Solution at Low Concentrations: A Fluorescence Study
pH (low) insertion peptide (pHLIP) is a 36-residue peptide derived from the third transmembrane helix of the membrane protein bacteriorhodopsin. The hydrophobicity of this peptide makes it prone to aggregation...
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Article
Lipid Membranes and Reactions at Lipid Interfaces: Theory, Experiments, and Applications
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Article
Role of Coulombic Repulsion in Collisional Lipid Transfer Among SMA(2:1)-Bounded Nanodiscs
Styrene/maleic acid (SMA) and related copolymers are attracting great interest because they solubilise membrane proteins and lipids to form polymer-encapsulated nanodiscs. These nanodiscs retain a lipid-bilaye...
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Article
Journal of Membrane Biology: Biophysics
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Article
Fluorierte Tenside und native Nano discs für die Membranproteinforschung
Integral membrane proteins are important but challenging research subjects. Various membrane mimetics have been developed that should preserve the native structure and function of membrane proteins while rende...
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Article
Modulating bilayer mechanical properties to promote the coupled folding and insertion of an integral membrane protein
Bilayer mechanical properties are not only of crucial importance to the mechanism of action of mechanosensation in lipid membranes but also affect preparative laboratory tasks such as membrane-protein refoldin...
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Article
Protein folding in membranes
Separation of cells and organelles by bilayer membranes is a fundamental principle of life. Cellular membranes contain a baffling variety of proteins, which fulfil vital functions as receptors and signal trans...