24 Result(s)
within
S Sanchez
Microbiology
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Article
Correction to: The starch-binding domain as a tool for recombinant protein purification
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Article
Animal and human RNA viruses: genetic variability and ability to overcome vaccines
RNA viruses, in general, exhibit high mutation rates; this is mainly due to the low fidelity displayed by the RNA-dependent polymerases required for their replication that lack the proofreading machinery to co...
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Chapter
Genetics and Biochemistry of Sporulation in Endospore-Forming Bacteria (Bacillus): A Prime Example of Developmental Biology
The development of spores represents one of the main morphogenetic processes in spore-forming bacterial groups, involving more than 150 genes and a variety of sigma factors. These factors control not only the ...
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Chapter
The Incredible Path of Myxobacteria Towards Aggregative Multicellularity
When reviewing developmental biology, it is imperative to mention the main prokaryote model for the study of mechanisms involved in cell growth, cell differentiation and morphogenesis, Myxobacteria. These Gram...
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Chapter
Effects of Cyanobacterial Metabolites on Other Bacterial Phyla and in the Morphogenesis, Viability, and Biochemistry of Eukaryotes
Prokaryotes were the earliest life forms on Earth but, although they originated an estimated 2 billion years before eukaryotic cells, both prokaryotes and eukaryotes share the same basic molecular mechanisms, ...
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Article
The starch-binding domain as a tool for recombinant protein purification
Recombinant protein purification with affinity tags is a widely employed technique. One of the most common tags used for protein purification is the histidine tag (Histag). In this work, we use a tandem starch-bi...
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Article
A single residue mutation abolishes attachment of the CBM26 starch-binding domain from Lactobacillus amylovorus α-amylase
Starch is degraded by amylases that frequently have a modular structure composed of a catalytic domain and at least one non-catalytic domain that is involved in polysaccharide binding. The C-terminal domain fr...
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Article
Sugar fermentation by Fusarium oxysporum to produce ethanol
As a first step in the research on ethanol production from lignocellulose residues, sugar fermentation by Fusarium oxysporum in oxygen-limited conditions is studied in this work. As a substrate, solutions of arab...
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Article
Generation of aroma compounds from Ditaxis heterantha by Saccharomyces cerevisiae
Ditaxis heterantha, a plant of the Euphorbiaceae family, is growing wild in the semiarid regions of Mexico. The seed endosperm contains yellow pigments (carotenoids). By high-pressure liquid chromatography the to...
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Article
Novel method for aroma recovery from the bioconversion of lutein to β-ionone by Trichosporon asahii using a mesoporous silicate material
In this work, we report on the synthesis and ability of the mesoporous material MCM-41 to adsorb the norisoprenoid β-ionone. This compound, with a violet aroma note, can be produced from lutein by the yeast Trich...
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Article
Production of tobacco aroma from lutein. Specific role of the microorganisms involved in the process
A mixed culture formed by Bacillus sp. and Geotrichum sp. produced tobacco aroma compounds from the carotenoid lutein through the formation of the intermediate β-ionone. Both microorganisms can grow independentl...
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Article
Ex vivo diffusion of albendazole and its sulfoxide metabolite into Ascaris suum and Fasciola hepatica
The current experiments compare the pattern of ex vivo uptake (diffusion) of albendazole (ABZ) and albendazole sulfoxide (ABZSO) by Ascaris suum and Fasciola hepatica. Specimens of A. suum and F. hepatica were c...
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Article
Copper but not iron limitation increases astaxanthin production by Phaffia rhodozyma in a chemically defined medium
The influence of copper (0–32 μM) and iron (0–108 μM) on growth and astaxanthin production by Phaffia rhodozyma was studied. Copper below 3.2 μM increased the astaxanthin content of the cells (from 220 to 287 μg ...
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Article
Citrate, a possible precursor of astaxanthin in Phaffia rhodozyma: influence of varying levels of ammonium, phosphate and citrate in a chemically defined medium.
The influence of ammonium, phosphate and citrate on astaxanthin production by the yeast Phaffia rhodozyma was investigated. The astaxanthin content in cells and the final astaxanthin concentration increased upon...
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Article
Bioconversion of lutein to products with aroma
A residual mud sample from the marigold flower dehydration process was screened and 19 putative colonies were isolated for their ability to degrade lutein in a chemically defined medium supplemented with mari...
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Article
Glucose repression of anthracycline formation in Streptomyces peucetius var. caesius
The effect of glucose on growth and anthracycline production by Streptomyces peucetius var. caesius was examined in a chemically defined medium. Glucose concentrations above 100 mM inhibited anthracycline synthe...
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Article
Influence of carbon and nitrogen sources on Flavobacteriumgrowth and zeaxanthin biosynthesis
Growth and production of zeaxanthin by Flavobacterium sp were studied using different carbon and nitrogen sources in a chemically defined medium. The best growth was supported by sucrose, but glucose yielded simi...
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Article
The production of xylitol from d-xylose by fermentation with Hansenula polymorpha
We have analysed the influence of the initial pH of the medium and the quantity of aeration provided during the batch fermentation of solutions of d-xylose by the yeast Hansenula polymorpha (34438 ATCC). The ini...
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Article
Anthracyclines: isolation of overproducing strains by the selection and genetic recombination of putative regulatory mutants of Streptomycespeucetius var. caesius
In Streptomyces peucetius var. caesius, the production of anthracyclines was suppressed either by 330 mM d-glucose or 25 mM phosphate. In addition, the anthracycline doxorubicin and the glucose analogue 2-deoxygl...
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Article
Physiology of lipase formation inPenicillium candidum
Penicillium candidum grew and produced lipase in a culture medium supplemented with 0.2% olive oil. Significant enzyme production required the presence of olive, oil and was prevented by cycloheximide. Polyacryl...
