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Article
Is endocytosis by caveolae dependent on dynamin?
The large GTPase dynamin has a crucial role in endocytosis, working at the neck of clathrin-coated pits to drive vesicular scission. Until recently, dynamin was believed to regulate endocytosis through caveola...
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Article
Open AccessApicobasal RNA asymmetries regulate cell fate in the early mouse embryo
The spatial sorting of RNA transcripts is fundamental for the refinement of gene expression to distinct subcellular regions. Although, in non-mammalian early embryogenesis, differential RNA localisation presag...
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Article
Open AccessIntegrative omics identifies conserved and pathogen-specific responses of sepsis-causing bacteria
Even in the setting of optimal resuscitation in high-income countries severe sepsis and septic shock have a mortality of 20–40%, with antibiotic resistance dramatically increasing this mortality risk. To devel...
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Article
Open AccessSugar transporter Slc37a2 regulates bone metabolism in mice via a tubular lysosomal network in osteoclasts
Osteoclasts are giant bone-digesting cells that harbor specialized lysosome-related organelles termed secretory lysosomes (SLs). SLs store cathepsin K and serve as a membrane precursor to the ruffled border, t...
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Author Correction: Volume electron microscopy
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Publisher Correction: Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis
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Article
Volume electron microscopy
Life exists in three dimensions, but until the turn of the century most electron microscopy methods provided only 2D image data. Recently, electron microscopy techniques capable of delving deep into the struct...
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Chapter
Epithelial Mechanosensing at Cell-Cell Contacts and Tight Junctions
This chapter focuses on the mechanosensitive properties of epithelial tissues. Epithelia experience a range of mechanical forces arising both intrinsically from their constituent cells and extrinsically from f...
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Article
Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis
Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis and cancer invasion. Unlike cargo-specific clathrin-mediated endocytosis, the clathrin- and dynamin-independent...
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Article
Open AccessPublisher Correction: Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis
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Article
Open AccessNicotinamide riboside attenuates age-associated metabolic and functional changes in hematopoietic stem cells
With age, hematopoietic stem cells (HSC) undergo changes in function, including reduced regenerative potential and loss of quiescence, which is accompanied by a significant expansion of the stem cell pool that...
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Article
Open AccessHigh intraluminal pressure promotes vascular inflammation via caveolin-1
The aetiology and progression of hypertension involves various endogenous systems, such as the renin angiotensin system, the sympathetic nervous system, and endothelial dysfunction. Recent data suggest that va...
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Article
Key principles and methods for studying the endocytosis of biological and nanoparticle therapeutics
Endocytosis is a critical step in the process by which many therapeutic nanomedicines reach their intracellular targets. Our understanding of cellular uptake mechanisms has developed substantially in the past ...
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Article
Open AccessCavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation
Caveolae are spherically shaped nanodomains of the plasma membrane, generated by cooperative assembly of caveolin and cavin proteins. Cavins are cytosolic peripheral membrane proteins with negatively charged i...
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Article
Caveolin-1 Ablation Imparts Partial Protection Against Inner Retinal Injury in Experimental Glaucoma and Reduces Apoptotic Activation
Retinal ganglion cell degeneration is a characteristic feature of glaucoma, and accordingly, protection of these cells constitutes a major therapeutic objective in the disease. Here, we demonstrate the key inf...
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Article
Open AccessIn vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation
The skeletal muscle T-tubule is a specialized membrane domain essential for coordinated muscle contraction. However, in the absence of genetically tractable systems the mechanisms involved in T-tubule formatio...
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Article
Open AccessReactivation of Myc transcription in the mouse heart unlocks its proliferative capacity
It is unclear why some tissues are refractory to the mitogenic effects of the oncogene Myc. Here we show that Myc activation induces rapid transcriptional responses followed by proliferation in some, but not a...
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Protocol
Live Confocal Imaging of Zebrafish Notochord Cells Under Mechanical Stress In Vivo
The zebrafish is a vertebrate model suited to the exploration of cell biology within a whole organism. Hypotheses in cell mechanics can be tested by using the zebrafish notochord as a manipulable experimental ...
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Article
Open AccessAn Abl-FBP17 mechanosensing system couples local plasma membrane curvature and stress fiber remodeling during mechanoadaptation
Cells remodel their structure in response to mechanical strain. However, how mechanical forces are translated into biochemical signals that coordinate the structural changes observed at the plasma membrane (PM...
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Article
Open AccessIdentification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis
Caveolae are specialized domains of the plasma membrane. Formation of these invaginations is dependent on the expression of Caveolin-1 or -3 and proteins of the cavin family. In response to stress, caveolae di...
