5 Result(s)
within
Rainer Löw
Life Sciences
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Chapter
Nonradioactive Detection of Nucleic Acids with Biotinylated Probes
Methods for nonradioactive detection of nucleic acids have replaced 32P-based techniques in many laboratories. Improvements in sensitivity allow the detection of single-copy genes in Southern blots and low abunda...
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Article
Salt stress induces an increased expression of V-type H+-ATPase in mature sugar beet leaves
In the halotolerant sugar beet co-expression of V-ATPase and a vacuolar Na+/H+-antiporter provides a mechanism for vacuolar salt sequestration. To analyze salt-induced changes in the expression of the vacuolar H+
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Protocol
Nonradioactive Northern Blotting
Recently, nonradioactive techniques for detection of DNA and RNA have been developed (1,2). The most commonly used labels are “digoxigenin,” “fluorescein,” and “biotin” which are linked through a spacer to a nucl...
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Protocol
Nonradioactive Northern Blotting of RNA
Technical improvements of methods used for the nonradioactive detection of nucleic acids have replaced the 32P-based techniques in many laboratories. The most commonly used labels are digoxigenin, fluorescein, an...
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Protocol
The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations
Today the treatment of inherited diseases holds a major field in gene therapy, and γ -retroviral vectors are often the preferred tool for stable introduction of the therapeutic gene(s) into the host cell genom...
