3 Result(s)
-
Protocol
Nonradioactive Northern Blotting
Recently, nonradioactive techniques for detection of DNA and RNA have been developed (1,2). The most commonly used labels are “digoxigenin,” “fluorescein,” and “biotin” which are linked through a spacer to a nucl...
-
Protocol
Nonradioactive Northern Blotting of RNA
Technical improvements of methods used for the nonradioactive detection of nucleic acids have replaced the 32P-based techniques in many laboratories. The most commonly used labels are digoxigenin, fluorescein, an...
-
Protocol
The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations
Today the treatment of inherited diseases holds a major field in gene therapy, and γ -retroviral vectors are often the preferred tool for stable introduction of the therapeutic gene(s) into the host cell genom...
