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Article
Open AccessElemental changes in the hippocampal formation following two different formulas of ketogenic diet: an X-ray fluorescence microscopy study
The main purpose of the following study was the determination of elemental changes occurring within hippocampal formation as a result of high-fat and carbohydrate-restricted ketogenic diet (KD). To realize it...
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Article
Safe biotechnology. Part 6. Safety assessment, in respect of human health, of microorganisms used in biotechnology
The assessment of microorganisms in respect to human health is an important step for the introduction of new natural and genetically modified production strains to biotechnology. This report outlines the poten...
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Article
Safe biotechnology (5). Recommendations for safe work with animal and human cell cultures concerning potential human pathogens
The benefits of using animal or human cell cultures have been clearly demonstrated in diagnostic and therapeutic research and in their application for manufacturing. Cell cultures serve as a tools for the prod...
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Article
Safe biotechnology (4). Recommendations for safety levels for biotechnological operations with microorganisms that cause diseases in plants
The Working Party on Safety in Biotechnology of the European Federation of Biotechnology has proposed a classification of microorganisms that cause diseases in plants. In this paper appropriate safety levels a...
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Chapter
Bioluminescent REC A Mutants of Rhizobium as Model Organisms in Risk Assessment Studies
A major concern about the deliberate release of genetically modified microorganisms (GEMMOs) is that the released organism exerts a potential disruptive effect on the biotic community of its target eco-system....
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Chapter
Squeezed States and Quadratic Hamiltonians: A Wigner Distribution Approach
For some time now there has been much interest in a special class of quantum states of the radiation field known as squeezed coherent states or simply squeezed states1–5. In fact the first experimental observatio...
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Chapter
Plasmid Interactions in Rhizobium; Incompatibility Between Symbiotic Plasmids
Plasmid profiles were determined for twenty four strains of R. trifolii. All the strains harboured at least three plasmids and as many as eight were present in some strains. Southern hybridisation with cloned R. ...
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Chapter
Advances in the Genetics of Free-Living and Symbiotic Nitrogen Fixing Bacteria
It is evident that the knowledge about biological nitrogen fixation was enormously increased when genetic systems in free-living and symbiotic nitrogen fixing bacteria were developed and used for the analysis ...
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Chapter
Mutagenesis
Bacterial plasmids are autonomously replicating mini chromosomes (replicons) which contain genetic information for many different properties, including the production of toxins and adhesion antigens, resistanc...
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Chapter
Basic Methods
Gene transfer in bacteria occurs by three main processes: transformation and transfection, which involve the direct uptake of free DNA by bacteria; transduction, which involves bacteriophage mediated transmiss...
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Chapter
Gene Cloning
Genetic recombination is the fundamental biological process of exchange of genetic information between different chromosomes. Its constant activity during evolution has enabled the large number of spontaneousl...
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Article
A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria
We have developed a new vector strategy for the insertion of foreign genes into the genomes of gram negative bacteria not closely related to Escherichia coli. The system consists of two components: special E. col...