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Article
Detection of human telomerase activity by telomerase TRAP — ELISA assay
Objective: Telomeric repeat amplification protocol (TRAP) is now a conventional assay for detecting telomerase activity. However, this method presents problems related to tedious quantifying, rad...
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Article
A Novel Replication-competent Adenovirus CNHK500 in the Treatment of Heptocellular Carcinoma In Vitro
Objective: To evaluate the therapeutic efficacy of replicative adenovirus CNHK500 in the treatment of hepatocellular carcinoma. Methods: Virus proliferation assay, cell viability assay and West...
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Article
Replication-selective Oncolytic Adenovirus CNHK300 in the Treatment of Breast Cancer Cell Lines in vitro
Objective: To evaluate the tumor selectivity and therapeutic efficiency of replication-competent adenovirus CNHK300 on human breast cancer cells. Methods: RT-PCR was used to detect the hTERT mRNA activity in v...
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Article
Monoclonal antibodies as therapeutic agents in oncology and antibody gene therapy
Antibodies as therapeutic agents are mostly used in oncology, as illustrated by their applications in lymphoma, breast cancer or colorectal cancer. This review provides a brief historical sketch of the develop...
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Article
A novel strategy for cancer treatment: Targeting cancer stem cells
Cancer stem cell/tumor-initiating cell (CSC/TIC) is a subclass of cancer cells possessing parts of properties of normal stem cell. It has a high capacity of proliferation and plays a pivotal role in tumor recu...
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Article
Capsid modification of adeno-associated virus and tumor targeting gene therapy
Targeting is critical for successful tumor gene therapy. The adeno-associated virus (AAV) has aroused wide concern due to its excellent advantages over other viral vectors in gene therapy. AAV has a broad infe...
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Article
Open AccessWnt/beta-catenin signaling in embryonic stem cell converted tumor cells
Embryonic stem cells (ESCs) are pluripotent stem cells and can form tumors containing cells from all three germ layers. Similarities between pluripotent stem cells and malignant tumor cells have been identifie...
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Article
Scalable manufacturing methodologies for improving adeno-associated virus-based pharmaprojects
Adeno-associated virus (AAV) is a promising viral vector and meets most requirements of being a safe biological agent. However, the commercialization of AAV has been hampered due to the limitation of large-sca...
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Article
Open AccessIntegrated analysis of miRNA, gene, and pathway regulatory networks in hepatic cancer stem cells
Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. HCC has a poor prognosis associated with tumor recurrence and drug resistance, which has been attributed to the existence of hep...
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Article
Open AccessThe combination of dendritic cells-cytotoxic T lymphocytes/cytokine-induced killer (DC-CTL/CIK) therapy exerts immune and clinical responses in patients with malignant tumors
The clinical trials using immunotherapy have been performed for the treatment of variety of malignant tumors. However, large-scale meta-analysis of combined DC-CTL/CIK therapy on immune and clinical response i...
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Chapter
Therapeutic Cancer Vaccines
Cancer is one of the major leading death causes of diseases. Prevention and treatment of cancer is an important way to decrease the incidence of tumorigenesis and prolong patients’ lives. Subversive achievemen...
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Article
Open AccessMonitoring checkpoint inhibitors: predictive biomarkers in immunotherapy
Immunotherapy has become the fourth cancer therapy after surgery, chemotherapy, and radiotherapy. In particular, immune checkpoint inhibitors are proved to be unprecedentedly in increasing the overall survival...
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Article
Open AccessModified CAR T cells targeting membrane-proximal epitope of mesothelin enhances the antitumor function against large solid tumor
Mesothelin (MSLN) is an attractive antigen for chimeric antigen receptor (CAR) T therapy and the epitope selection within MSLN is essential. In this study, we constructed two types of CARs targeting either reg...
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Article
Open AccessCorrection: Modified CAR T cells targeting membrane-proximal epitope of mesothelin enhances the antitumor function against large solid tumor
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
Open AccessChimeric antigen receptor T cells engineered to secrete CD40 agonist antibodies enhance antitumor efficacy
Although chimeric antigen receptor (CAR)-T cell therapy has been remarkably successful for haematological malignancies, its efficacy against solid tumors is limited. The combination of CAR-T cell therapy with ...
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Article
Phase I clinical trial of EGFR-specific CAR-T cells generated by the piggyBac transposon system in advanced relapsed/refractory non-small cell lung cancer patients
This phase I clinical trial is designed to assess the safety and feasibility of the epidermal growth factor receptor (EGFR) chimeric antigen receptor (CAR) T-cell generated by the piggyBac transposon system in...
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Article
Open AccessAntitumor activity of Z15-0-2, a bispecific nanobody targeting PD-1 and CTLA-4
The combination of programmed cell death protein 1 (PD-1) and cytotoxic T lymphocyte antigen 4 (CTLA-4) antibodies has potential for enhancing clinical efficacy. We described the development and antitumor acti...
