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Article
A Novel Fluorescent FRET Hairpin Probe Switch for aflD Gene Detection in Real Fermented Soybean Paste
The development of detection method for aflatoxin gene cluster in Aspergillus flavus and Aspergillus parasiticusis is an effective approach to prevent the widespread of aflatoxins, which are considered as extreme...
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Article
Development of an Indirect Competitive ELISA Kit for the Detection of Soybean Allergenic Protein Gly m Bd 28K
The complementary DNA (cDNA) sequence of Gly m Bd 28K was expressed in Escherichia coli BL21 (DE3) as an inclusion body under the induction of 1.0 mmol/L of isopropyl β-D-thiogalactoside (IPTG). The inclusion bod...
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Article
Development of an Indirect Competitive ELISA Kit for the Rapid Detection of Benzopyrene Residues
This paper presents the generation of monoclonal antibodies (mAbs) with high specificity against benzopyrene (BaP) through cell fusion procedures, and the development of mAb-based indirect competitive enzyme-l...