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  1. Article

    Open Access

    Characterization of a novel aspartic protease from Trichoderma asperellum for the preparation of duck blood peptides

    A novel aspartic protease gene (TaproA1) from Trichoderma asperellum was successfully expressed in Komagataella phaffii (Pichia pastoris). TaproA1 showed 52.8% amino acid sequence identity with the aspartic prote...

    Yibin Xue, Qiaojuan Yan, Xue Li, Zhengqiang Jiang in Applied Microbiology and Biotechnology (2024)

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  2. No Access

    Article

    Global transcriptomic analysis of functional oligosaccharide metabolism in Pediococcus pentosaceus

    Lactic acid bacteria (LAB) are important in food fermentation and may enhance overall host health. Previous studies to explore LAB metabolism mainly focused on the genera Lacticaseibacillus and Lactococcus. Pedio...

    Dong Han, Ran Shi, Qiaojuan Yan, Yuqin Shi… in Applied Microbiology and Biotechnology (2021)

  3. No Access

    Article

    Biochemical characterization of a novel α-L-fucosidase from Pedobacter sp. and its application in synthesis of 3′-fucosyllactose and 2′-fucosyllactose

    Fucosyllactoses have gained much attention owing to their multiple functions, including prebiotic, immune, gut, and cognition benefits. In this study, human milk oligosaccharide (HMO) 2′-fucosyllactose (α-L-Fu...

    Ran Shi, Junwen Ma, Qiaojuan Yan, Shaoqing Yang… in Applied Microbiology and Biotechnology (2020)

  4. No Access

    Article

    Metabolic engineering of Clostridium tyrobutyricum for n-butanol production from sugarcane juice

    Clostridium tyrobutyricum is a promising organism for butyrate and n-butanol production, but cannot grow on sucrose. Three genes (scrA, scrB, and scrK) involved in the sucrose catabolic p...

    Jianzhi Zhang, Le Yu, Mengmeng Xu… in Applied Microbiology and Biotechnology (2017)

  5. No Access

    Article

    Modulating the function of a β-1,3-glucanosyltransferase to that of an endo-β-1,3-glucanase by structure-based protein engineering

    A glycoside hydrolase (GH) family 17 β-1,3-glucanosyltransferase (RmBgt17A) from Rhizomucor miehei CAU432 (CGMCC No. 4967) shared very low sequence homology (∼20 % identity) with that of other β-1,3-glucanases, d...

    Zhen Qin, Qiaojuan Yan, Shaoqing Yang… in Applied Microbiology and Biotechnology (2016)

  6. Article

    Open Access

    Genome sequence and transcriptome analyses of the thermophilic zygomycete fungus Rhizomucor miehei

    The zygomycete fungi like Rhizomucor miehei have been extensively exploited for the production of various enzymes. As a thermophilic fungus, R. miehei is capable of growing at temperatures that approach the upper...

    Peng Zhou, Guoqiang Zhang, Shangwu Chen, Zhengqiang Jiang, Yanbin Tang… in BMC Genomics (2014)

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  7. No Access

    Article

    Characterization of two novel family 12 xyloglucanases from the thermophilic Rhizomucor miehei

    Two novel glycoside hydrolase (GH) family 12 xyloglucanase genes (designated RmXEG12A and RmXEG12B) were cloned from the thermophilic fungus Rhizomucor miehei. Both genes contained open reading frames of 729 bp e...

    Shuang Song, Yanbin Tang, Shaoqing Yang… in Applied Microbiology and Biotechnology (2013)

  8. No Access

    Article

    High-level expression of a specific β-1,3-1,4-glucanase from the thermophilic fungus Paecilomyces thermophila in Pichia pastoris

    In this study, a novel β-1,3-1,4-glucanase gene (designated as PtLic16A) from Paecilomyces thermophila was cloned and sequenced. PtLic16A has an open reading frame of 945 bp, encoding 314 amino acids. The deduced...

    Chengwei Hua, Qiaojuan Yan, Zhengqiang Jiang… in Applied Microbiology and Biotechnology (2010)