6 Result(s)
-
Article
Open AccessHuman extrahepatic and intrahepatic cholangiocyte organoids show region-specific differentiation potential and model cystic fibrosis-related bile duct disease
The development, homeostasis, and repair of intrahepatic and extrahepatic bile ducts are thought to involve distinct mechanisms including proliferation and maturation of cholangiocyte and progenitor cells. Thi...
-
Article
Open AccessPrime editing for functional repair in patient-derived disease models
Prime editing is a recent genome editing technology using fusion proteins of Cas9-nickase and reverse transcriptase, that holds promise to correct the vast majority of genetic defects. Here, we develop prime e...
-
Article
Open AccessLGR5 marks targetable tumor-initiating cells in mouse liver cancer
Cancer stem cells (CSCs) or tumor-initiating cells (TICs) are thought to be the main drivers for disease progression and treatment resistance across various cancer types. Identifying and targeting these rare c...
-
Article
Open AccessUltra-thin fluorocarbon foils optimise multiscale imaging of three-dimensional native and optically cleared specimens
In three-dimensional light microscopy, the heterogeneity of the optical density in a specimen ultimately limits the achievable penetration depth and hence the three-dimensional resolution. The most direct appr...
-
Article
Open AccessLipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells
Wnt signalling proteins are essential for culture of human organ stem cells in organoids, but most Wnt protein formulations are poorly active in serum-free media. Here we show that purified Wnt3a protein is in...
-
Article
Tissue-specific mutation accumulation in human adult stem cells during life
Stem cells of the liver, colon and small intestine gradually accumulate mutations throughout life at a similar rate even though cancer incidence varies greatly among these tissues.
