17 Result(s)
within
Michele A. Pellegrino
Biomedicine
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Article
The monoclonal antibody CR11-351 discriminates HLA-A2 variants identified by T cells
Serologic and immunochemical assays have shown that the monoclonal antibody (MoAb) CR11-351 recognizes a determinant expressed by HLA-A2 and A28 alloantigens. The MoAb CR1 1-351 blocks the cytotoxicity of some...
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Chapter
Immunochemical Analysis of the Antigenic Profile of Human Melanoma Cells with Monoclonal Antibodies
Tumor immunologists have been investigating the antigenic profile of cancer cells in order to define cellular changes induced by malignant transformation. Characterization of these changes may aid our understa...
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Article
Stimulation of human T lymphocytes by PHA-activated autologous T lymphocytes: Analysis of the role of Ia-like antigens with monoclonal antibodies
Human T lymphocytes activated with PHA express Ia-like antigens and acquire the ability to stimulate autologous T lymphocytes in mixed lymphocyte reaction. This reaction is immunological in nature since it has...
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Article
The monoclonal xenoantibody Q6/64 recognizes a determinant expressed by certain gene products of the A and B loci of the HLA region
A combination of serological (cytotoxicity, binding assay, lysostrip) and immunochemical (indirect immunoprecipitation, sequential immunoprecipitation, two-dimensional gel electrophoresis) assays have shown th...
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Article
Immunochemical analysis of anti-HLA-A2, HLA-A3, and HLA-B27 xenoantisera elicited with hybrids between human and murine cells
Rabbits were immunized with hybrids constructed with human and murine cells. Serological and immunochemical studies showed that xenoantiserum 1595 is operationally specific for HLA-A2, xenoantisera 0806 and 07...
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Article
The free and the β2-microglobulin-associated heavy chains of HLA-A, B alloantigens share the antigenic determinant recognized by the monoclonal antibody Q1/28
Serological and immunochemical assays have shown that the monoclonal antibody Q1/28 recognizes an antigenic determinant which is expressed on the heavy chain of subsets of HLA-A, B antigens and is distinct fro...
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Article
Cross-reactivity between human and murine lymphocyte antigens
The anti-H-2 alloantiserum D-32 [(BlO.A(2R) × C3H.SW) anti-C3H] is cytolytic to human lymphocytes. Fab2 blocking assays, indirect immunoprecipitation and sequential immunoprecipitation experiments showed that the...
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Chapter
Structural Analyses of HLA-DR Antigens
There has been considerable progress in the characterization of HLA-DR antigens even though these human counterparts of murine and guinea pig Ir gene products were identified only a few years ago. The information...
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Article
Effect of polyclonal and monoclonal HLA-DR xenoantibodies on xenogeneic mixed lymphocyte reactions
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Article
Xenoantisera to human DR antigens: Serological and immunochemical characterization
Antibodies to DR antigens were detected using serological and immunochemical tests in sera from rabbits and goats immunized with cultured human B-lymphoid cells mixed with an anti-T-cell xenoantiserum or with ...
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Article
Structural relationship between W4/W6 antigens and other surface markers on cultured human lymphoid cells as determined by the ‘lysostrip’ method
The structural relationship of histocompatibility antigens W4 and W6 with other surface markers on cultured human lymphoid cells of the B type has been investigated, utilizing the lysostrip technique. W4 and W...
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Chapter
Human DR (Ia-Like) Antigens: Biological and Molecular Profile
The functions that a cell of the immune system performs may be mediated through particular surface macromolecules. Although specific cell-surface markers are known to be associated with various functionally di...
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Article
Evidence for linkage between HLA antigens and receptors for complement components C3b and C3d in human-mouse hybrids
A linkage between HLA-A and HLA-B antigens and receptors for complement components C3b and C3d has been demonstrated by means of human-mouse somatic cell hybridization. In 26 hybrid clones formed between the h...
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Article
Linkage between the B-cell specific receptor for monkey red blood cells and HL-A antigens in man-mouse hybrids
The established human lymphoid cell lines 6410 and WI-L2 exhibit the recently discovered receptor for monkey red blood cells (MRBC). This receptor is specific for B cells. These lymphoid lines were fused with ...
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Article
Activation of the complement system by H-2 alloantisera: Differential susceptibility to lysis of thymocytes and lymphocytes
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Article
Association of HL-A antigens andβ 2-microglobulin at the cellular and molecular level
Surfaces of cultured human lymphoid cells RPMI 1788, RPMI 4098, RPMI 8866, Raji, and WI-L2 were found to contain bothβ 2-microglobulin (β 2-μ) and HL-A determinants when tested by direct complement-dependent cyto...
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Article
Cross-reactivity between human and murine lymphocyte antigens: III. Reactivity of H-2 allo- and xenoantisera with human lymphoid cells
H-2 alloantisera and antimouse lymphocyte xenoantisera react with 14%–100% of human lymphocytes from a panel of at least 80 unrelated people. Population and family studies did not reveal HL-A specificity of su...
