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Article
Genotype diversity and antibiotic resistance risk in Aeromonas hydrophila in Sichuan, China
Sichuan is a significant aquaculture province in China, with a total aquaculture output of 1.72 × 106 tons in 2022. One of the most significant microorganisms hurting the Sichuan aquaculture is Aeromonas hydrophi...
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Protocol
Transiently Induce RNA Silencing in Plants Using a Tobacco Necrosis Virus A (TNV-A)-Based dsRNA Production System
Transgenic expression of hairpin RNA or artificial microRNA is widely used for genetic studies in plant science. However, induction of RNA silencing by transgenic method may have a problem when studying essent...
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Protocol
Isolation of Double-Stranded RNAs by Lithium Chloride Fractionation
This procedure provides a comprehensive method for isolating double-stranded RNA (dsRNA) that relies on the different solubility of various nucleic acids in lithium chloride (LiC1). The approach offers several...
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Article
Serum Cu, Zn and IL–1β Levels May Predict Fetal Miscarriage Risk After IVF Cycles: A Nested Case–Control Study
To explore the association between serum-related indicators (levels of inflammatory cytokines and essential trace elements) and miscarriage risk among infertile women undergoing assisted reproductive technique...
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Article
Open AccessNeuroligin 2 governs synaptic morphology and function through RACK1-cofilin signaling in Drosophila
Neuroligins are transmembrane cell adhesion proteins well-known for their genetic links to autism spectrum disorders. Neuroligins can function by regulating the actin cytoskeleton, however the factors and mech...
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Article
Study on the Correlation Between Regulatory Proteins of N6-methyladenosine and Oxidative Damage in Cadmium-induced Renal Injury
As a common environmental heavy metal pollutant, cadmium has been well evidenced to cause kidney damage; yet, the underlying mechanisms are still not fully clarified. In this study, cell viability of human ren...
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Article
A single-cell transcriptome atlas profiles early organogenesis in human embryos
The early window of human embryogenesis is largely a black box for developmental biologists. Here we probed the cellular diversity of 4–6 week human embryos when essentially all organs are just laid out. On th...
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Article
Open AccessTurnip mosaic virus manipulates DRM2 expression to regulate host CHH and CHG methylation for robust infection
DNA methylation is an important epigenetic marker for the suppression of transposable elements (TEs) and the regulation of plant immunity. However, little is known how RNA viruses counter defense such antivira...
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Article
Identification and characterization of L-type lectin receptor-like kinases involved in Glycine max–Phytophthora sojae interaction
Soybean contains a group of 64 L-type lectin receptor-like kinases. Three LecRKs were involved in the interactions with Phytophthora sojae and Bradyrhizobium diazoefficiens.
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Article
Open AccessPP2Acα promotes macrophage accumulation and activation to exacerbate tubular cell death and kidney fibrosis through activating Rap1 and TNFα production
Macrophage accumulation and activation play an essential role in kidney fibrosis; however, the underlying mechanisms remain to be explored. By analyzing the kidney tissues from patients and animal models with ...
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Article
An improved draft genome sequence of hybrid Populus alba × Populus glandulosa
Populus alba × P. glandulosa clone 84 K, derived from South Korea, is widely cultivated in China and used as a model in the molecular research of woody plants because of high gene transformation efficiency. Here,...
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Article
Open AccessPoplar aquaporin PIP1;1 promotes Arabidopsis growth and development
Root hydraulic conductance is primarily determined by the conductance of living tissues to radial water flow. Plasma membrane intrinsic proteins (PIPs) in root cortical cells are important for plants to take u...
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Article
Open AccessThe miR-204-5p/FOXC1/GDF7 axis regulates the osteogenic differentiation of human adipose-derived stem cells via the AKT and p38 signalling pathways
Human adipose-derived stem cells (hADSCs) are stem cells with the potential to differentiate in multiple directions. miR-204-5p is expressed at low levels during the osteogenic differentiation of hADSCs, and i...
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Article
Open AccessThe HIV protease inhibitor Saquinavir attenuates sepsis-induced acute lung injury and promotes M2 macrophage polarization via targeting matrix metalloproteinase-9
Imbalance of macrophage polarization plays an indispensable role in acute lung injury (ALI), which is considered as a promising target. Matrix metalloproteinase-9 (MMP-9) is expressed in the macrophage, and ha...
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Protocol
Using GBP Nanotrap to Restore Autophagy in the Rab5/Vps21 Mutant by Forcing Snf7 and Atg17 Interaction
Protein–protein interactions are important for physiology performance. Green fluorescent protein (GFP) is a widely used protein tag to show protein localization in vivo. GFP binding protein (GBP) is a specific...
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Article
Genetic architecture of leaf morphological and physiological traits in a Populus deltoides ‘Danhong’ × P. simonii ‘Tongliao1’ pedigree revealed by quantitative trait locus analysis
Understanding the genetic architecture of leaf morphological and physiological traits will help plant breeders develop high biomass poplar genotypes. Quantitative trait locus (QTL) studies combining next-gener...
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Article
Physiological and metabolic responses of Salix sinopurpurea and Salix suchowensis to drought stress
Physiological and metabolic analysis showed the difference in drought tolerance and revealed the common and specific metabolic regulations in Salix sinopurpurea and Salix suchowensis.
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Article
An essential role for Arabidopsis Trs33 in cell growth and organization in plant apical meristems
Trafficking protein particle (TRAPP) complexes subunit gene AtTrs33 plays an important role in kee** apical meristematic activity and dominance in Arabidopsis.
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Article
Open AccessGenome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance
Drought is a major environmental constraint to plant growth, development and productivity. Compared with most willows that are generally susceptible to drought, the desert willow Salix psammophila has extraordina...
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Article
PagMYB216 is involved in the regulation of cellulose synthesis in Populus
Cellulose accounts for most of the structural material in secondary cell walls. Although the synthesis of cellulose has been extensively investigated, the regulation of cellulose synthesis in woody plants rema...