24 Result(s)

Article

Modification of transcriptional factor ACE3 enhances protein production in Trichoderma reesei in the absence of cellulase gene inducer

Trichoderma reesei is one of the best-known cellulolytic organisms, producing large quantities of a complete set of extracellular cellulases and hemicellulases for the degradation of lignocellulosic substances. H...

Yun Luo, Mari Valkonen, Raymond E. Jackson… in Biotechnology for Biofuels (2020)

Article

Development of a low-cost cellulase production process using Trichoderma reesei for Brazilian biorefineries

During the past few years, the first industrial-scale cellulosic ethanol plants have been inaugurated. Although the performance of the commercial cellulase enzymes used in this process has greatly improved ove...

Simo Ellilä, Lucas Fonseca, Cristiane Uchima, Junio Cota… in Biotechnology for Biofuels (2017)

Article

Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei

The filamentous fungus Trichoderma reesei has tremendous capability to secrete over 100 g/L of proteins and therefore it would make an excellent host system for production of high levels of therapeutic proteins a...

Christopher P. Landowski, Eero Mustalahti, Ramon Wahl… in Microbial Cell Factories (2016)

Article

The effects of extracellular pH and of the transcriptional regulator PACI on the transcriptome of Trichoderma reesei

Extracellular pH is one of the several environmental factors affecting protein production by filamentous fungi. Regulatory mechanisms ensure that extracellular enzymes are produced under pH-conditions in which...

Mari Häkkinen, Dhinakaran Sivasiddarthan, Nina Aro… in Microbial Cell Factories (2015)

Article

Comparison of intracellular and secretion-based strategies for production of human α-galactosidase A in the filamentous fungus Trichoderma reesei

Trichoderma reesei is known as a good producer of industrial proteins but has hitherto been less successful in the production of therapeutic proteins. In order to elucidate the bottlenecks of heterologous protein...

Wesley Smith, Jussi Jäntti, Merja Oja, Markku Saloheimo in BMC Biotechnology (2014)

Article

Screening of candidate regulators for cellulase and hemicellulase production in Trichoderma reesei and identification of a factor essential for cellulase production

The soft rot ascomycetal fungus Trichoderma reesei is utilized for industrial production of secreted enzymes, especially lignocellulose degrading enzymes. T. reesei uses several different enzymes for the degradat...

Mari Häkkinen, Mari J Valkonen, Ann Westerholm-Parvinen… in Biotechnology for Biofuels (2014)

Article

Re-annotation of the CAZy genes of Trichoderma reesei and transcription in the presence of lignocellulosic substrates

Trichoderma reesei is a soft rot Ascomycota fungus utilised for industrial production of secreted enzymes, especially lignocellulose degrading enzymes. About 30 carbohydrate active enzymes (CAZymes) of T. reesei ...

Mari Häkkinen, Mikko Arvas, Merja Oja, Nina Aro, Merja Penttilä… in Microbial Cell Factories (2012)

Article

The effects of disruption of phosphoglucose isomerase gene on carbon utilisation and cellulase production in Trichoderma reesei Rut-C30

Cellulase and hemicellulase genes in the fungus Trichoderma reesei are repressed by glucose and induced by lactose. Regulation of the cellulase genes is mediated by the repressor CRE1 and the activator XYR1. T. r...

M Carmen Limón, Tiina Pakula, Markku Saloheimo, Merja Penttilä in Microbial Cell Factories (2011)

Article

Erratum to: Discovery of a new tyrosinase-like enzyme family lacking a C-terminally processed domain: production and characterization of an Aspergillus oryzae catechol oxidase

Chiara Gasparetti, Greta Faccio, Mikko Arvas… in Applied Microbiology and Biotechnology (2011)

Article

Correction: Corrigendum: Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina)

Nat. Biotechnol. 26, 553–560 (2008); published online 4 May 2008; corrected after print 9 October 2008. In the version of this article initially published, an author's name was misspelled as Barbote. The corre...

Diego Martinez, Randy M Berka, Bernard Henrissat, Markku Saloheimo… in Nature Biotechnology (2008)

Article

Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina)

Trichoderma reesei is the main industrial source of cellulases and hemicellulases used to depolymerize biomass to simple sugars that are converted to chemical intermediates and biofuels, such as ethanol. We assem...

Diego Martinez, Randy M Berka, Bernard Henrissat, Markku Saloheimo… in Nature Biotechnology (2008)

Article

Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or indu...

Brigitte Gasser, Markku Saloheimo, Ursula Rinas… in Microbial Cell Factories (2008)

Article

Physiological evaluation of the filamentous fungus Trichoderma reesei in production processes by marker gene expression analysis

Biologically relevant molecular markers can be used in evaluation of the physiological state of an organism in biotechnical processes. We monitored at high frequency the expression of 34 marker genes in batch,...

Jari J Rautio, Michael Bailey, Teemu Kivioja, Hans Söderlund… in BMC Biotechnology (2007)

Article

Heterologous expression of isotopically labeled Trichoderma reesei tyrosinase 2 in Pichia pastoris

Ann Westerholm-Parvinen, Maija-Liisa Mattinen… in Microbial Cell Factories (2006)