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Article
Temperature immutability of adenyl cyclase-coupled β adrenergic recptors
OBSERVATIONS from several laboratories have suggested that the well established classification of adrenergic receptors into α and β subtypes is not immutable. Several groups have reported that in isolated perfuse...
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Chapter
Membrane-Bound Hormone Receptors
Receptors are those cellular structures with which biologically active hormones and drugs first interact. Physiologically these receptors perform two essential functions. The first is that of recognition of a ...
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Article
Biochemical characterization of the BETA-adrenergic receptor of the frog erythrocyte
The beta-adrenergic receptor which is coupled to adenylate cyclase in the frog erythrocycte plasma membrane provides a convenient model system for probing the molecular characteristics of an adenylate cyclase ...
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Article
Pure β-adrenergic receptor: the single polypeptide confers catecholamine responsiveness to adenylate cyclase
The β-adrenergic receptor binding subunits from frog erythrocytes, hamster lung and guinea pig lung have been purified to apparent homogeneity and in all cases reside on a single polypeptide. Insertion of the ...
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Chapter
The β-Adrenergic Receptor:Elucidation of Its Molecular Structure
Neurotransmitters and drugs interact with specific receptors in order to produce a cellular response. This recognition of specific agonists by receptors is the first step in an amplification process resulting ...
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Article
Reconstitution of the β-adrenergic receptor
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Article
A role for Ni in the hormonal stimulation of adenylate cyclase
The best understood system for transduction of extracellular messages into intracellular signals is the hormone receptor-coupled adenylate cyclase. In such systems receptors are functionally coupled to the enz...
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Article
Cloning of the gene and cDNA for mammalian β-adrenergic receptor and homology with rhodopsin
The adenylate cyclase system, which consists of a catalytic moiety and regulatory guanine nucleotide-binding proteins, provides the effector mechanism for the intracellular actions of many hormones and drugs1. Th...
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Article
Light-dependent phosphorylation of rhodopsin by β-adrenergic receptor kinase
The structural components involved in transduction of extracellular signals as diverse as a photon of light im**ing on the retina or a hormone molecule im**ing on a cell have been highly conserved. These c...
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Chapter
Molecular Mechanisms of β-Adrenergic Receptor Desensitization
Desensitization or adaptation is well known in biological regulation. Also referred to as tachyphylaxis, tolerance or refractoriness, it is most commonly observed as a loss of cellular responsiveness to a neur...
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Article
Cross-talk between cellular signalling pathways suggested by phorbol-ester-induced adenylate cyclase phosphorylation
Receptor-mediated activation of both adenylate cyclase and phosphatidylinositide hydrolysis systems occurs through guanine nucleotide regulatory proteins and ultimately leads to specific activation of either c...
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Article
An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleotide regulatory proteins
Plasma membrane receptors for hormones, drugs, neurotransmit-ters and sensory stimuli are coupled to guanine nucleotide regulatory proteins. Recent cloning of the genes and/or cDNAs for several of these recep...
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Chapter
D1 and D2 Dopamine Receptors: Identification by Photoaffinity Labeling and Purification by Affinity Chromatography
In an attempt to characterize the receptors which mediate the physiological actions of dopamine, we have developed several probes for the characterization of both the D1 and D2 subtypes of dopamine receptors. To ...
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Chapter
Biochemical Properties of D1 and D2 Dopamine Receptors
The physiological action of dopamine are mediated by two distinct subtypes of receptors, D1 and D2 dopamine receptors. D1-receptors are linked to stimulation of adenylate cyclase whereas D2-receptors inhibit the ...
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Chapter
Isolation And Biochemical Characterization Of The D1 And D2 Dopamine Receptors
The catecholamine, dopamine, exerts physiologic effects in both the central nervous system and the periphery. Dopamine systems in the central nervous system (CNS) have been implicated in several neurologic and...
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Chapter
The β-Adrenergic Receptor Kinase: Role in Homologous Desensitization in S49 Lymphoma Cells
Phosphorylation of the β-adrenergic receptor (BAR) is closely associated with homologous desensitization of the B-adrenergic receptor-coupled adenylate cyclase system. Homologous desensitization and receptor p...
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Chapter
Regulation of the Adenylate Cyclase Signalling Pathway: Potential Role for the Phosphorylation of the Catalytic Unit by Protein Kinase A and Protein Kinase C
We have investigated the phosphorylation of the pure catalytic unit of adenylate cyclase by cyclic AMP-dependent protein kinase (PKA) and Ca2+/phospholipid-dependent protein kinase (PKC). The catalytic unit of ad...
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Article
Removal of phosphorylation sites from the β2-adrenergic receptor delays onset of agonist-promoted desensitization
Eukaryotic cells have evolved a variety of mechanisms for dampening their responsiveness to hormonal stimulation in the face of sustained activation. The mechanisms for such processes, collectively referred to...
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Article
The genomic clone G-21 which resembles a β-adrenergic receptor sequence encodes the 5-HT1A receptor
The recent cloning of the complementary DNAs and/or genes for several receptors linked to guanine nucleotide regulatory proteins including the adrenergic receptors (α1, α2A, α2B, β1, β2)1–7 several subtypes of t...
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Article
Molecular cloning and expression of the gene for a human D1 dopamine receptor
THE diverse physiological actions of dopamine are mediated by its interaction with two basic types of G protein-coupled receptor, D1 and D2, which stimulate and inhibit, respectively, the enzyme adenylyl cyclase1
