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2,385 Result(s)
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Article
Regulative Erscheinungen bei der Planarienregeneration unter anormalen Bedingungen
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Injury to primary cultures of rat heart endothelial cells by hypoxia and glucose deprivation
Primary cultures of rat heart endothelial cells were subjected to simulated conditions of ischemia: hypoxia and glucose deprivation for 4 and 24 hr. Cellular injury was evaluated by measuring changes in viabil...
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Reduction of cell injury in hypoxic cultures of rat myocardial cells by methylprednisolone
An in vitro model to study myocardial cell injury was developed with primary monolayer cultures of rat myocardial cells. Two important conditions associated with myocardial ischemia were simulated by depriving...
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Behavior of primary and serially transplanted estrogen-dependent renal carcinoma cells in monolayer and in collagen gel culture
When estrogen is present in culture medium, enzyme-dissociated cells from estrogen-induced primary renal tumors in Syrian hamsters and from 1st to 4th serially transplanted carcinomas in monolayer culture cont...
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Cellular injury of primary cultures of rat myocytes incubated in calcium-free medium followed by recovery in calcium
Primary monolayer cultures of rat myocardial cells were used to study the cellular injury that occurs when calcium is reintroduced after a period of calcium depletion. Cultures were treated with a calcium-free...
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Two types of glomus cell in the rat carotid body as revealed by α-bungarotoxin binding
Horseradish peroxidase (HRP)-conjugated α-bungarotoxin (αBgt) was used to localize αBgt-acetylcholine receptor sites in the rat carotid body. Two types of glomus cell were differentiated on the basis of the st...
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Cell injury of cultured rat myocardial cells after reoxygenation of hypoxic cultures in the presence and absence of calcium
Primary cultures of rat myocytes were deprived of oxygen (approximately 5 mm Hg, pO2) for 2 h in the presence or absence of calcium and were subsequently incubated for different time periods under normoxic (appro...
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Dopamine β-hydroxylase-like immunoreactivity in the rat and cat carotid body: a light and electron microscopic study
Immunocytochemical localization of dopamine β-hydroxylase (DBH) was used to study the synthesis and storage sites of norepinephrine (noradrenaline) in the rat and cat carotid bodies. In the rat carotid body so...
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Gene map** on human metaphase chromosomes by in situ hybridization with 3H, 35S, and 32P labeled probes and transmission electron microscopy
A method based on in situ hybridization, autoradiography and transmission electron microscopy for map** genes on human metaphase chromosomes is presented. Successful map** of the tandemly repeated rDNA gen...
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Article
Transferrin is a major mouse milk protein and is synthesized by mammary epithelial cells
We have identified a major mouse milk protein as transferrin (Tf) using immunoprecipitation, 2-dimensional electrophoresis, Ouchterlony diffusion and V-8 protease digests. We show that Tf is synthesized by mam...
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Synthesis of laminin-related polypeptides in oocytes, eggs and early embryos of the amphibian Pleurodeles waltlii
In the amphibian Pleurodeles waltlii, lamininrelated polypeptides (amphibian-LN) are present in the extracellular matrix underlying the blastocoel roof of gastrulating embryos. Immunoprecipitation with affinity-p...
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Establishment and characterization of a continuous murine uterine cervix cancer cell line metastatic to lymph nodes and lungs
The murine uterine cervix cancer (MUCC) cell line was derived from a chemically induced Kunming mouse uterine cervix cancer (U27) and maintained in culture on solid substrates for over 100 passages. Cultures w...
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Altered low density lipoprotein receptor regulation is associated with cholesteryl ester accumulation in simian virus 40 transformed rodent fibroblast cell lines
Several SV40 transformed REF52 cell lines were found to accumulate 5 to 10 times more cholesteryl esters compared to their parent line REF52 when cultured in 10% serum. Under this culture condition, the choles...
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A study of cell interactions involved in Pleurodeles waltlii epidermal differentiation
A polyclonal antibody (SP-2) has been produced, which recognizes antigens expressed in epidermal cells of Pleurodeles waltlii embryos. The antigens appear first at the end of gastrulation in the external surface ...
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Inversely related evolution of growth hormone and prolactin secretions in long-term tissue cultures of human pituitary adenomas from acromegalic patients
Pituitary tumoral tissue from 20 acromegalic patients was cultured for up to 120 d in a medium containing 5 nM cortisol. In all cultures, growth hormone (GH) release decreased. At the beginning of the culture, pr...
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Article
Production of monoclonal antibodies against avian LHRH-I
Production of antibodies against peptides or poorly antigenic proteins by conventional methods often requires either large quantities of the native immunogen or some chemical modification to increase their ant...
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Wallerian degeneration in the peripheral nervous system: participation of both Schwann cells and macrophages in myelin degradation
This study examined the role of Schwann cells and hematogenous macrophages in myelin degradation and Ia antigen expression during Wallerian degeneration of rodent sciatic nerve. To identify and distinguish bet...
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Adult cardiac muscle cells in long-term serum-free culture: Myofibrillar organization and expression of myosin heavy chain isoforms
A culture system for adult rat cardiac muscle cells has been established without exposure of cells to serum at any step of the procedure. The methodology has been standardized and optimized to obtain better qu...
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Lithium induces dorsal-type migration of mesodermal cells in the entire marginal zone of urodele amphibian embryos
The effect of lithium (Li+) on gastrulation movements was investigated during the development of the urodele amphibianPleurodeles waltl. Attention was focused on mesodermal cell migration. Under conditions of Li+
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Epidermal growth factor, DNA synthesis and human hepatocytes