1,961 Result(s)
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Article
A phylogenetic comparison of mutation spectra
Spectra of induced mutations resulting in obligate organoauxotrophy are compared at different phylogenetic levels from bacteria to higher plants. It appears that the spectra of mutants concerned with basic cel...
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Thiamine mutants of the crucifer, Arabidopsis
In higher plants the only obligate organoauxotrophic mutants available are concerned with the synthesis of thiamine, vitamin B1. In Arabidopsis 65 mutants, representing four loci controlling four different steps ...
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A study of genetic polymorphisms of milk β-lactoglobulin, αS1-casein, β-casein, and κ-casein in five dairy breeds
Gene frequencies of the milk β-lactoglobulin, αS1-casein, β-casein, and κ-casein loci have been estimated from 1663 cows of five dairy breeds. Departure from Hardy-Weinberg equilibrium was found only in the κ-cas...
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Article
Nucleotide sequence divergence among DNA fractions of different syngens of Tetrahymena pyriformis
The magnitude of the differences in base sequence of DNA fractions derived from different syngens of the ciliated protozoan Tetrahymena pyriformis was investigated. Each DNA was fractionated into unique and repea...
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A virus-specified mechanism for the prevention of multiple infection—T7- and T3-mutual and superinfection exclusion
Co-and superinfection of cells with T3/T7 result in exclusion (mutual or superinfection exclusion). The exclusion mechanism is also directed against homologous (or identical) virus. Exclusion is established af...
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E. coli membranes become permeable to ions following T7-virus-infection
Infection of E. coli with the viruses T7 or T3 leads to a dramatic efflux of potassium ions. This ion efflux is caused by the virus particle since no concomitant protein synthesis is required. T7 mutants carrying...
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Article
Correlation between structural variation and activity of murine kidney β-galactosidase: Implications for genetic control
Two closely linked regulatory genes have been reported to control activity levels of β-galactosidases in murine tissues. The specific effects of these genes on murine glycolipid metabolism have not been elucid...
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Article
Early T7 gene expression
The mode of transcription of early T7 genes starting from one promotor region and generating a unique polycistronic RNA species suggests the appearance of equimolar amounts of the monocistronic species after R...
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Article
The half-life of mRNA in Saccharomyces cerevisiae
The decay kinetics of mRNA was studied in a yeast temperature-sensitive mutant, ts136, which is defective in cytoplasmic RNA production at 37° C. The disappearance of the synthetic capacity of mRNA was determined...
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Article
Radiation sensitivity of messenger RNA
Messenger RNA function is inactivated by irradiation with ultraviolet light. A unit length mRNA (in bases) is 2–3 times more sensitive than a unit length of DNA (in base pairs) with respect to the inactivation...
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Structural relatedness of mouse lactate dehydrogenase isozymes, A4 (muscle), B4 (heart), and C4 (testis)
Three homotetrameric lactate dehydrogenase isozymes, LDH-M(A4), LDH-H(B4), and LDH-X(C4), from DBA/2J mice have been purified by affinity chromatography. The amino acid compositions of the subunits A, B, and C, b...
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Article
Nitrogen regulation of uricase synthesis in Neurospora crassa
Neurospora crassa can utilize various purine bases as a nitrogen source. Synthesis of the purine catabolic enzymes such as uricase requires induction by the intermediate uric acid and is also subject to nitrogen...
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Evidence for the transcription of physiologically inactive rat-liver nucleolar chromatin byEscherichia coli RNA polymerase
Rat-liver nucleoli (10-15 pg DNA) were digested with either 0.6 or 3 units of DNase I for various times (up to 1 h). RNA synthesis was then measured in the absence or presence ol 3 units ofEscherichia coli RNA po...
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Human liver alcohol dehydrogenase: ADHIndianapolis results from genetic polymorphism at the ADH 2 gene locus
New molecular forms of human liver alcohol dehydrogenase (ADH), collectively designated ADHIndianapolis (ADHInd), were recently discovered in 29% of liver specimens from Black Americans [Bosron, W. F., Li, T.-K.,...
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Polymorphism of human liver alcohol dehydrogenase: Identification of ADH2 2-1 and ADH2 2-2 phenotypes in the Japanese by isoelectric focusing
Liver homogenate-supernatants from most Japanese exhibit an “atypical” pH optimum for ethanol oxidation at pH 8.8 instead of 10.5, the “typical” pH-activity optimum. It has been proposed that atypical livers cont...
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Purification and characterization of mouse alcohol dehydrogenase from two inbred strains that differ in total liver enzyme activity
Alcohol dehydrogenase activity in mouse liver homogenate-supernatants is 1.7 times greater in the C57BL/10 strain than in the BALB/c strain, regardless of whether activity is expressed in units per gram liver,...
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Article
Pleiotropic mutants of Chinese hamster cells with altered cytidine 5′-triphosphate synthetase
Following chemical mutagenesis and multiple-step indirect selection, four clones of Chinese hamster V79 cells were isolated which exhibited auxotrophy for thymidine, deoxycytidine, or deoxyuridine but not for ...
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Suppression of defective-sporulation phenotypes by mutations in the major sigma factor gene (rpoD) of Bacillus subtilis
Mutations (crsA47 and crsA4) in the major sigma factor gene (rpoD) of Bacillus subtilis RNA polymerase have been found to be powerful intergenic suppressors of spoOB, spoOE, spoOF, spoOK and spoIIG mutations. The...
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Genetically determined differences in liver alcohol dehydrogenase activity in male rats
Alcohol dehydrogenase (EC 1.1.1.1) activity was measured in liver extracts from one outbred and three inbred strains of rats. Strain-specific differences in enzyme activity were observed in the adult male rats...
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Gene map** on human metaphase chromosomes by in situ hybridization with 3H, 35S, and 32P labeled probes and transmission electron microscopy
A method based on in situ hybridization, autoradiography and transmission electron microscopy for map** genes on human metaphase chromosomes is presented. Successful map** of the tandemly repeated rDNA gen...