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937 Result(s)
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Article
Gene map** on human metaphase chromosomes by in situ hybridization with 3H, 35S, and 32P labeled probes and transmission electron microscopy
A method based on in situ hybridization, autoradiography and transmission electron microscopy for map** genes on human metaphase chromosomes is presented. Successful map** of the tandemly repeated rDNA gen...
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Article
Nuclear receptor sites for vitamin D-soltriol in midbrain and hindbrain of Siberian hamster (Phodopus sungorus) assessed by autoradiography
Autoradiograms were prepared from midbrains and hindbrains of male and female Siberian hamsters (Phodopus sungorus), kept under short-day or long-day illumination, after injection of tritium-labeled 1,25-dihydrox...
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Article
Three new DNA helicases fromSaccharomyces cerevisiae
At least six DNA helicases have been identified during fractionation of extracts from the yeastSaccharomyces cerevisiae. Three of those, DNA helicases B, C, and D, have been further purified and characterized. DN...
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Article
HeLa D98/AH-2 studied by chromosome painting and conventional cytogenetical techniques
Metaphase chromosomes of HeLa D98/AH-2 cells were studied by GTG-banding, NOR-silver staining and in situ hybridization using libraries specific for each human chromosome as probes (chromosome painting). The s...
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Vitamin D3 (soltriol) nuclear receptors in abdominal scent gland and skin of Siberian hamster (Phodopus sungorus) localized by autoradiography and immunohistochemistry
In vivo autoradiography with [3H]1,25-dihydroxycholecalciferol (vitamin D, soltriol) and immuno-staining with antibodies to vitamin D receptor were applied to identify specific binding sites in the abdominal scen...
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Article
Histochemical demonstration of copper in LEC rat liver
Livers of LEC rats were histochemically stained for copper according to the modified Timm's method, which includes trichloroacetic acid (TCA) treatment. TCA pretreatment was effective in removing zinc and iron...
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Article
Use of Phaseolus vulgaris leukoagglutinating lectin in histochemical and blotting techniques: a comparison of digoxigenin- and biotin-labelled lectins
An increase in the number of β 1,6 branches of the trimannosyl core of asparagine-linked oligosaccharides has been shown to be directly correlated with the metastatic potential of cultured tumour cells. The Phase...
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Article
Relationship between cellular DNA synthesis, PCNA expression and sex steroid hormone receptor status in the develo** mouse ovary, uterus and oviduct
The proliferative activities of the different cellular compartments of the develo** mouse ovary, uterus, and oviduct were studied by radioautographic assessment of DNA synthesis with [3H]-thymidine labeling and...
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Article
Argyrophilic nucleolar organizer regions
Silver staining techniques developed to demonstrate argyrophilic nucleolar organizer regions (Ag-NORs) have been widely applied in a variety of cell kinetic studies, using the mean number of AgNORs in tumour c...
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Article
Human gamma X satellite DNA: an X chromosome specific centromeric DNA sequence
The cosmid clone, CX16-2D12, was previously localized to the centromeric region of the human X chromosome and shown to lack human X-specific α satellite DNA. A 1.2 kb EcoRI fragment was subcloned from the CX16...
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Article
Applications of immunogold and lectin-gold labeling in tumor research and diagnosis
Immunohistochemistry and carbohydrate histochemistry have had an enormous impact on both tumor research and diagnosis. In particular, immunogold labeling has provided significant advantages over classical fluo...
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Article
Localization of the Drosophila checkpoint control protein Bub3 to the kinetochore requires Bub1 but not Zw10 or Rod
We report here the isolation and molecular characterization of the Drosophila homolog of the mitotic checkpoint control protein Bub3. The Drosophila Bub3 protein is associated with the centromere/kinetochore of ...
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Article
Characterisation of endothelin-1-related protein in human adrenal cortex and in cortical lesions
Endothelin (ET)-1 is a 21-amino acid peptide with potent vasopressor and vasoconstrictive properties. Biochemical studies suggest that this peptide occurs in the adrenal cortex, where it appears to influence ...
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Article
Homolog pairing and meiotic progression in Coprinus cinereus
We have used fluorescence in situ hybridization to examine homolog pairing during the synchronous meiosis of the basidiomycete Coprinus cinereus. Using spread preparations of meiotic nuclei, we confirmed previous...
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The Role of the p35/cdk5 Kinase in Cortical Development
Extensive studies on the mouse mutant reeler have revealed many of the fundamental characteristics of neocortical development (Caviness and Rakic 1978; Caviness 1982; Caviness et al. 1988). The finding of another...
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Article
Differences in the distribution of synemin, paranemin, and plectin in skeletal muscles of wild-type and desmin knock-out mice
Mice lacking the gene encoding for the intermediate filament protein desmin have a surprisingly normal myofibrillar organization in skeletal muscle fibers, although myopathy develops in highly used muscles. I...
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Article
Association of connexin36 with zonula occludens-1 in HeLa cells, βTC-3 cells, pancreas, and adrenal gland
The PDZ domain-containing protein zonula occludens-1 (ZO-1), a well-established component of tight junctions, has recently been shown to interact with various connexin proteins that form gap junctions. We inve...
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Article
Adhesion, actin cytoskeleton organisation and the spreading of colon adenocarcinoma cells induced by EGF are mediated by α2β1 integrin low clustering through focal adhesion kinase
Both epidermal growth factor (EGF) and the extracellular matrix components have been implicated in the pathobiology of adenocarcinomas by somewhat poorly understood mechanisms. We have addressed this problem ...
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Article
A decade of CDK5
Since it was identified a decade ago, cyclin-dependent kinase 5 (CDK5) has emerged as a crucial regulator of neuronal migration in the develo** central nervous system. CDK5 phosphorylates a diverse list of s...
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Article
Stage-specific chromosomal association of Drosophila dMBD2/3 during genome activation
The Drosophila gene dMBD2/3 encodes a protein with significant homologies to the mammalian methyl-DNA binding proteins MBD2 and MBD3. These proteins are essential components of chromatin complexes involved in ep...