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8,508 Result(s)
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Article
Direct evidence for models of heterosis provided by mutants of Arabidopsis blocked in the thiamine pathway
Auxotrophic mutants genetically blocked at different steps of the thiamine pathway dramatically demonstrate the biochemical mechanism of hybrid vigor due to simple and perfect dominance at two unlinked loci. H...
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Influence of glass microbeads on growth, activity and morphological changes of Bacillus megaterium
Cells of Bacillus megaterium growing in the presence of glass microbeads with average diameters of 29 and 53 μm were frequently filamentous and sometimes reached lengths of 600 μm. Some of the filaments were nons...
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Synchronization of 9L rat brain tumor cells by centrifugal elutriation
Asynchronous 9L cells were separated into relatively homogeneously-sized populations using centrifugal elutriation with both a conventional collection method and a long collection method. A substantial increas...
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Characterization of the separation properties of the beckman elutriator system
The role of fluid flow in the elutriation process was visualized by pum** dye solution through the Beckman JE-6 elutriator rotor. Three major fluid flow disturbances were observed in the separation chambers,...
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Quantitative production of xylitol from D-xylose by a high-xylitol producing yeast mutant Candida tropicalis HXP2
Xylitol was produced as a metabolic by-product by a number of yeasts when grown on medium containing D-xylose as carbon and energy sources. Among the yeast strains tested, a mutant strain of Candida tropicalis (H...
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Plantlet regeneration from mesophyll protoplasts of Digitalis lanata Ehrh.
Protoplasts were isolated from the mesophyll of Digitalis lanata enzymatically and cultured in a liquid regeneration medium (D2a). Protoplast division occurred at a rate of approximately 30%. Mature cell colonies...
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Evidence for the transcription of physiologically inactive rat-liver nucleolar chromatin byEscherichia coli RNA polymerase
Rat-liver nucleoli (10-15 pg DNA) were digested with either 0.6 or 3 units of DNase I for various times (up to 1 h). RNA synthesis was then measured in the absence or presence ol 3 units ofEscherichia coli RNA po...
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Book reviews
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Article
Glucoamylase covalently coupled to porous glass
Glucoamylase (EC 3.2.1.3) was immobilized to alkylamine porous glass with glutaraldehyde. The choice and pretreatment of carrier and conditions for immobilization have been investigated. The immobilized enzyme...
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Homogeneous immunoassays
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Chloroplast DNA and cytoplasmic male-sterility
This paper deals with the relationship of chloroplast DNA (ctDNA) and cytoplasmic malesterility (CMS) of maize, wheat and rape. Intramolecular heterogeneity and fragment patterns of DNA digested with restricti...
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Different effects of selenium on cyclic AMP metabolism in hepatoma cells and normal liver cells
Sodium selenite administered to normal and hepatoma (HA)-bearing mice sc (2 mg/kg) or ip (1 mg/kg) led to a significant increase in cyclic AMP (cAMP) level and a decrease in cAMP phosphodiesterase (PDE) activi...
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Abstracts
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Article
Wall-bound proteins of pollen tubes after self- and cross-pollination in Lilium longiflorum
Wall-bound proteins of Lilium longiflorum pollen tubes grown in vivo constitute 20–27% of the dry matter. Twenty-two-twenty-six percent of these proteins are NaCl soluble. Wall-bound proteins of in vivo pollen tu...
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Selenium effects on camp metabolism in hepatoma cells and normal liver cells
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Article
Biophoton emission
The phenomenon of ultraweak photon emission from living systems was further investigated in order to elucidate the physical properties of this radiation and its possible source. We obtained evidence that the l...
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Two dimensional analysis of thylakoid membrane polypeptides of a barley mutant
One dimensional SDS-PAGE and two dimensional electrophoresis of the thylakoid membrane proteins of a barley mutant and its original variety were carried out. It was found that (1) approximately 20 bands could ...
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Glucoamylase and α-amylase production by immobilized Aspergillus niger
Aspergillus niger mycelia or spores were immobilized in calcium alginate gel beads and employed for production of glucoamylase and α-amylase by repeated batch process. The immobilized mycelium produced lower enz...
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Regional variation of cancer mortality incidence and its relation to selenium levels in China
The epidemiological relationship between selenium level and age-adjusted human cancer mortality (incidence) was studied in 24 regions located in eight provinces of China. Statistically significant inverse corr...
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Preliminary screening of some fungal strains for protein upgrading of sugar-beet pulp
Fourteen fungal strains were grown on sugar-beet pulp (SBP) in a semisolid state with the view of evaluating their capabilities in increasing the protein content of SBP. The highest increases were obtained withSp...