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Article
The Necessary Site for Initiation of RNA Synthesis in the 3′-Noncoding Region of Classical Swine Fever Virus Genome
Classical swine fever virus (CSFV) is the causative agent of swine fever, which represents an economically important disease in hogs. We previously made a prediction about the recognition sites of replication ...
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Article
Molecular cloning and characterization of a novel ice gene from Capsella bursa-pastoris
A new ice gene (designated as Cbice53, an inducer of CBF expression) was cloned from Capsella bursa-pastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA of Cbice53 was 1811 bp long, with a 14...
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Article
Cloning and Characterization of a Novel Mouse Short-Chain Dehydrogenases/Reductases cDNA, mHsdl2, Encoding a Protein with an SDR Domain and an SCP2 Domain
The short-chain dehydrogenases/reductases (SDRs) play an important role in the body's metabolism. We have cloned a novel mouse SDR cDNA, which encodes a deduced HSD-like protein with a conserved SDR domain and...
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Article
A correlation between radiation sensitivity and initial chromatid breaks in cancer cell lines revealed by Calyculin A-induced premature condensation
Three human malignancy cell lines were irradiated with 60Co γ-rays. Initial chromatid breaks were measured by using the chemically induced premature chromosome condensation technique. Survival curves of cells exp...
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Article
Identification of a novel human MAST4 gene, a new member of human microtubule associated serine/threonine kinase family
Human protein kinases make up a large superfamily of homologous proteins, which are related by virtue of their kinase domains (also known as catalytic domains). Here, we report the cloning and characterization...
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Protocol
In Silico Prediction of Peptide-MHC Binding Affinity Using SVRMHC
The binding between peptide epitopes and major histocompatibility complex (MHC) proteins is a major event in the cellular immune response. Accurate prediction of the binding between short peptides and class I ...
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Article
Construction of T-vectors for the direct, unidirectional cloning, and analysis of PCR-amplified promoters
The amplification and cloning of promoters are regularly employed procedures to study the mechanism of gene regulation. In the present study we developed a method to construct T-vectors used for the direct and...
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Article
Hydrogen peroxide (H2O2) and methyl-β-cyclodextrin (MβCD) down regulate caveolin expression in human lens epithelial cells (HLECs)
Oxidative stimulation induced by hydrogen peroxide (H2O2) on human lens epithelial cells (HLECs) was performed to observe the effects on cell viability and caveolin expression, and cholesterol depletion in HLECs ...
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Article
Reversal of multidrug resistance of gastric cancer cells by downregulation of CIAPIN1 with CIAPIN1 siRNA
The overexpression of a new cytokine-induced apoptosis inhibitor 1 (CIAPIN1) gene has been shown previously to promote a multidrug resistant phenotype in gastric cancer cells through the upregulation of MDR1 a...
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Article
Characterization and expression analysis of two cotton genes encoding putative UDP-Glycosyltransferases
UDP-Glycosyltransferases (UGT) are a large family of enzymes, which catalyze the transfer of a sugar from an activated sugar donor to an acceptor molecule. Both in plants and in mammals, they are important in ...
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Article
Hypoxia induced HIF-1 accumulation and VEGF expression in gastric epithelial mucosa cells: Involvement of ERK1/2 and PI3K/Akt
Hypoxia is a common environmental stress that influences signaling pathways and cell function, which through initiates intracellular signaling pathways and hence leads to the activation of the transcription fa...
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Article
NF-κB modulates activation of the BMP-2 gene by trichostatin A
In this study, trichostatin A (TSA), a histone deacetylase inhibitor, increased the Bone morphogenetic protein-2 (BMP-2) mRNA level in a human osteoblasts line. Deletion analysis of the promoter region revealed t...
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Article
Inhibiting gene expression in vivo by virus-mediated small interfering RNA
Inhibiting gene expression in specific tissues and organs through intravenous injection would be the ultimately preferred method of disease therapy. Here, we report the successful delivery of lentivirus-mediat...
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Article
Transcriptional regulation of the human gene coding for proline-rich nuclear receptor coactivator (PNRC) by regulatory factor X (RFX1)
PNRC (Proline-rich Nuclear Receptor Coactivator) is a novel coactivator for multiple nuclear receptors. PNRC was previously identified using bovine SF-1 (steroidogenic factor 1) as the bait in a yeast two-hybr...
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Article
Establishment of a novel sensitive method for detecting methylation modification on DNA of escherichia coli cell
This study focused on finding a novel sensitive method to determine the methylation modification at DNA dam (GATC) sites in Escherichia coli. A new plasmid which contained three GATC sites recognized by restricti...
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Article
Genetic diversity of starch synthesis genes of Chinese maize (Zea mays L.) with SNAPs
Analysis of genetic diversity in maize populations is a very important step for understanding genetic structure and subsequently for genetic manipulations in maize breeding. Sh2, Bt2, Sh1, Wx1, Ae1 and Su1 involv...
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Article
Comparison of antioxidant capacity and phenolic compounds of berries, chokecherry and seabuckthorn
Antioxidant capacity and phenolic compounds (phenolic acids and anthocyanins) of four berry fruits (strawberry, Saskatoon berry, raspberry and wild blueberry), chokecherry and seabuckthorn were compared in the...
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Article
The use of the ccdB lethal gene for constructing a zero background vector in order to clone blunt-end PCR Products
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Article
Endostar, a recently introduced recombinant human endostatin, inhibits proliferation and migration through regulating growth factors, adhesion factors and inflammatory mediators in choroid-retinal endothelial cells
Retinal neovascularization is one of the leading causes for complete blindness in a number of diseases around the world. Endostar, a recently introduced recombinant human endostatin, has been considered as one...
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Article
Biochemical responses in gills of rainbow trout exposed to propiconazole
The aim of this study is to investigate the toxic effect of PCZ, a triazole fungicide commonly present in surface and ground water, on the ROS defense system and Na+-K+-ATPase in gills of rainbow trout exposed to...