34 Result(s)
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Lijie **ang
Life Sciences
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Article
The E2F family: a ray of dawn in cardiomyopathy
Cardiomyopathies are a group of heterogeneous diseases, characterized by abnormal structure and function of the myocardium. For many years, it has been a hot topic because of its high morbidity and mortality a...
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Open AccessTumour microenvironment programming by an RNA–RNA-binding protein complex creates a druggable vulnerability in IDH-wild-type glioblastoma
Patients with IDH-wild-type glioblastomas have a poor five-year survival rate along with limited treatment efficacy due to immune cell (glioma-associated microglia and macrophages) infiltration promoting tumou...
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Open AccessCorrection: Assessing immunogenicity of CRISPR‑NCas9 engineered strain against porcine epidemic diarrhea virus
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Low temperature and dark culture inhibited explant browning and promoted in vitro regeneration of Juglans mandshurica
Juglans mandshurica is an important component of Korean pine mixed and secondary forests in Northeast China and is also a precious timber and economic species. In this study, plant regeneration of J. mandshurica
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Open AccessAssessing immunogenicity of CRISPR-NCas9 engineered strain against porcine epidemic diarrhea virus
Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV), is an acute and highly infectious disease, resulting in substantial economic losses in the pig industry. Given that PEDV primar...
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QTL detection for internode diameter and its association with yield-related traits in wheat
Optimal stem structure affects stem strength and significantly contributes to wheat yield, for which internode diameter (ID) characteristics are considered important. To specify the genetic basis of wheat inte...
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Open AccessDifferent dietary starch patterns in low-protein diets: effect on nitrogen efficiency, nutrient metabolism, and intestinal flora in growing pigs
Protein releases amino acids faster than starch releases glucose in digestive tract of pigs fed low-protein (LP) diets. Poor synchronization of dietary glucose and amino acids supply leads to compromised nitro...
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Complete genomic sequence of Hibiscus latent Fort Pierce virus in a new host, Passilora edulis, in China
Hibiscus latent Fort Pierce virus (HLFPV) is a tobamovirus with a few species of the family Malvaceae as its natural host. Here, we reported the identification of HLFPV in passion fruit (Passilora edulis) in Chin...
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Open AccessStructural characterization of Platanthera ussuriensis chloroplast genome and comparative analyses with other species of Orchidaceae
The genus Tulotis has been classified into the genus Platanthera in the present taxonomic studies since the morphological characteristics of this genus is very similar to that of Platanthera. Platanthera ussurien...
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Open Access2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism
Overflow metabolism-caused acetate accumulation is a major problem that restricts industrial applications of various bacteria. 2,3-Butanediol (2,3-BD) synthesis in microorganisms is an ancient metabolic proces...
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Open AccessEfficiency comparison of B6(Cg)-Tyrc−2j /J and C57BL/6NTac embryos as hosts for the generation of knockout mice
Careful selection of the host embryo is critical to the efficient production of knockout (KO) mice when injecting mouse embryonic stem (mES) cells into blastocysts. B6(Cg)-Tyrc−2j/J (B6 albino) and C57BL/6NTac (B...
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Eliminating base-editor-induced genome-wide and transcriptome-wide off-target mutations
The fusion of CRISPR–Cas9 with cytidine deaminases leads to base editors (BEs) capable of programmable C-to-T editing, which has potential in clinical applications but suffers from off-target (OT) mutations. H...
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Open AccessGenome-wide analysis of differentially expressed mRNAs, lncRNAs, and circRNAs in chicken bursae of Fabricius during infection with very virulent infectious bursal disease virus
Infectious bursal disease virus (IBDV) causes acute, highly contagious, immunosuppressive, and lethal infectious disease in young chickens and mainly infects the bursa of Fabricius (BF). To investigate interac...
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Antifungal activity of an endophytic strain of Phomopsis sp. on Sclerotinia sclerotiorum, the causal agent of Sclerotinia disease
Ethyl acetate crude extract obtained from fermented cultures of Phomopsis sp. strain S4 (strain S4 extract) was effective in the control of mycelial growth and influenced to some extent sclerotia germination and ...
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Oral delivery of a Lactococcus lactis strain secreting bovine lactoferricin–lactoferrampin alleviates the development of acute colitis in mice
Ulcerative colitis (UC) is a chronic relapsing disease. Treatment of UC would benefit from specific targeting of therapeutics to the intestine. Previous studies have demonstrated that bovine lactoferricin and ...
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Open AccessLong noncoding RNA PCAT1, a novel serum-based biomarker, enhances cell growth by sponging miR-326 in oesophageal squamous cell carcinoma
Long noncoding RNAs (lncRNAs) play important roles in the development and progression of human cancers. The lncRNA prostate cancer-associated transcript 1 (PCAT1) has been reported to be involved in multiple h...
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Open AccessConstruction of Lactobacillus casei ghosts by Holin-mediated inactivation and the potential as a safe and effective vehicle for the delivery of DNA vaccines
Bacterial ghosts (BGs) are empty bacterial cell envelopes generated by releasing the cellular contents. In this study, a phage infecting Lactobacillus casei ATCC 393 (L. casei 393) was isolated and designated Lcb...
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APOBEC3 induces mutations during repair of CRISPR–Cas9-generated DNA breaks
The APOBEC-AID family of cytidine deaminase prefers single-stranded nucleic acids for cytidine-to-uridine deamination. Single-stranded nucleic acids are commonly involved in the DNA repair system for breaks ge...
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Open AccessEnhanced base editing by co-expression of free uracil DNA glycosylase inhibitor
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Open AccessHighly efficient and precise base editing in discarded human tripronuclear embryos
