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  1. No Access

    Article

    Improvement of human interferon alpha secretion by Lactococcus lactis

    To improve the secretion and expression of human interferon alpha 2b (IFN) in Lactococcus lactis, a synthetic pro-peptide, LEISSTCDA (LEISS), was fused to the N-terminus of IFN. This gave a higher secretion effic...

    Qiuxiang Zhang, ** Zhong, **aobo Liang, Wenjun Liu, Liandong Huan in Biotechnology Letters (2010)

  2. No Access

    Article

    Secretory Expression of Nattokinase from Bacillus subtilis YF38 in Escherichia coli

    Nattokinase producing bacterium, B. subtilis YF38, was isolated from douchi, using the fibrin plate method. The gene encoding this enzyme was cloned by polymerase chain reaction (PCR). Cytoplasmic expression of t...

    **aobo Liang, Shifang Jia, Yufang Sun, Meiling Chen, **uzhu Chen… in Molecular Biotechnology (2007)

  3. No Access

    Article

    Secretory expression of a heterologous nattokinase in Lactococcus lactis

    Nattokinase has been reported as an oral health product for the prevention of atherosclerosis. We developed a novel strategy to express a nattokinase from Bacillus subtilis in a live delivery vehicle, Lactococcus...

    **aobo Liang, Lixin Zhang, ** Zhong… in Applied Microbiology and Biotechnology (2007)

  4. No Access

    Article

    Purification and Characterization of Two Novel Antimicrobial Peptides Subpeptin JM4-A and Subpeptin JM4-B Produced by Bacillus subtilis JM4

    An antimicrobial peptides-producing strain was isolated from soil and identified as Bacillus subtilis JM4 according to biochemical tests and 16S rDNA sequence analysis. The corresponding antimicrobial peptides we...

    Shimei Wu, Shifang Jia, Dandan Sun, Meiling Chen, **uzhu Chen… in Current Microbiology (2005)

  5. No Access

    Article

    Expression of a human lactoferrin N-lobe in Nicotiana benthmiana with potato virus X-based agroinfection

    A DNA fragment encoding the N-terminal half (N-lobe) of the human lactoferrin (hLfN) gene has now been cloned into recombinant Potexvirus potato virus X (PVX) vector and expressed in Nicotiana benthmiana using ag...

    Yong Li, Yunfeng Geng, Houhui Song, Guangyu Zheng, Liandong Huan… in Biotechnology Letters (2004)