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    Secretory Expression of Nattokinase from Bacillus subtilis YF38 in Escherichia coli

    Nattokinase producing bacterium, B. subtilis YF38, was isolated from douchi, using the fibrin plate method. The gene encoding this enzyme was cloned by polymerase chain reaction (PCR). Cytoplasmic expression of t...

    **aobo Liang, Shifang Jia, Yufang Sun, Meiling Chen, **uzhu Chen in Molecular Biotechnology (2007)