538 Result(s)
within
Li** Sheng
Medicine & Public Health
Internal Medicine
Oncology
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Article
Apoptosis of human BEL-7402 hepatocellular carcinoma cells released by antisense H-ras DNA-in vitro and in vivo studies
Recent findings suggest that over-expression of activated H-ras inhibited apoptotic cell death by blocking the activity of apoptotic endonuclease(s). This study was designed using antisense H-ras oligodeoxynucleo...
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A ribozyme specifically suppresses transformation and tumorigenicity of Ha-ras-oncogene-transformed NIH/3T3 cell lines
In this study, the efficacy of an anti-ras ribozyme in reversing a transformed phenotype was investigated. A murine NIH/3T3-derived cell line, designated 2–12, contains an inducible Ha-ras oncogene, which is regu...
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Inhibition of tumor growth and metastasis of human breast cancer cells transfected with tissue inhibitor of metalloproteinase 4
We recently identified, cloned, and characterized a novel human tissue inhibitor of metalloproteinases-4, TIMP-4 (Greene et al., 1996). To determine if TIMP-4 can modulate the in vivo growth of human breast cance...
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Suppression of autocrine cell proliferation and tumorigenesis of human melanoma cells and fibroblast growth factor transformed fibroblasts by a kinase-deficient FGF receptor 1: evidence for the involvement of Src-family kinases
Basic Fibroblast Growth Factor (bFGF/FGF2) is thought to play a decisive role in malignant progression. Aberrant expression of bFGF causes constitutive autocrine activation of its cognate receptor and autonomo...
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Epstein-Barr virus in synergy with tumor-promoter-induced malignant transformation of immortalized human epithelial cells
It is difficult to study how Epstein-Barr virus (EBV) causes transformation of human epithelial cells. The major difficulty is that cultured human epithelial cells do not express EBV receptor (complement recep...
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Induced direct binding of the adapter protein Nck to the GTPase-activating protein-associated protein p62 by epidermal growth factor
The SH3-SH3-SH3-SH2 adapter protein Nck links receptor tyrosine kinases, such as EGF and PDGF receptors, to downstream signaling pathways, among which p21cdc42/rac-activated kinase cascade, Sos-activated Ras sign...
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Potential role for Cathepsin D in p53-dependent tumor suppression and chemosensitivity
Cathepsin D (CD), the major intracellular aspartyl protease, is a mediator of IFN-γ and TNF-α induced apoptosis. Using subtractive hybridization screening we isolated CD as an upregulated transcript in PA1 hum...
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Article
Downregulation of platelet-derived growth factor receptor-β in Shp-2 mutant fibroblast cell lines
The SH2-containing tyrosine phosphatase Shp-2 appears to function downstream of a variety of growth factor receptors and might play a positive role in cell proliferation. Here we report that expression of the ...
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Article
Shp-2 has a positive regulatory role in ES cell differentiation and proliferation
Shp-2 is a ubiquitously expressed tyrosine phosphatase with two SH2 domains. Homozygous mutant mice with a targeted deletion of 65 amino acid residues in the N-terminal SH2 domain of Shp-2 die in utero at mid-ges...
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TGF-β1 effects on proliferation of rat intestinal epithelial cells are due to inhibition of cyclin D1 expression
Transforming growth factor-beta 1 (TGF-β1) arrests intestinal epithelial cells (RIE-1 and IEC-6) in the G1 phase of the cell cycle and inhibits cyclin D1 expression. This report describes experiments designed ...
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The germinal center kinase (GCK)-related protein kinases HPK1 and KHS are candidates for highly selective signal transducers of Crk family adapter proteins
Adapter proteins function by mediating the rapid and specific assembly of multi-protein complexes during the signal transduction which guards proliferation, differentiation and many functions of higher eukaryo...
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Functional association of TGF-β receptor II with cyclin B
Utilizing the cytoplasmic tail of Transforming Growth Factor Receptor Type II (TGFβ RII) as bait in a yeast two hybrid system, we have identified human cyclin B2 as a direct physical partner of TGFβ RII. Analy...
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Transformation of intestinal epithelial cells by chronic TGF-β1 treatment results in downregulation of the type II TGF-β receptor and induction of cyclooxygenase-2
The precise role of TGF-β in colorectal carcinogenesis is not clear. The purpose of this study was to determine the phenotypic alterations caused by chronic exposure to TGF-β in non-transformed intestinal epit...
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Fatal mycobacteremia caused by Mycobacterium tuberculosis in a patient with acute leukemia
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Article
Mechanisms of apoptosis induced by the synthetic retinoid CD437 in human non-small cell lung carcinoma cells
The novel synthetic retinoid 6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalene carboxylic acid (CD437) has been shown to induce apoptosis in various tumor cell lines including human non-small cell lung carcino...
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Article
Prohibitin, a potential tumor suppressor, interacts with RB and regulates E2F function
The retinoblastoma tumor suppressor protein and its family members, p107 and p130, are major regulators of the mammalian cell cycle. They exert their growth suppressive effects at least in part by binding the ...
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Article
Induction of the TRAIL receptor KILLER/DR5 in p53-dependent apoptosis but not growth arrest
The TRAIL death receptor KILLER/DR5 is induced by DNA damaging agents in wild-type p53-expressing cells. Here we show that, unlike the p53-target CDK-inhibitor p21WAF1/CIP1, the TRAIL death receptor KILLER/DR5 is...
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Article
Role of basal calcium in the EGF activation of MAP kinases
The role of intracellular Ca2+ pools in the regulation of growth factor signal transduction pathways and mitogenesis is not well understood. We have examined the roles of basal and transiently mobilized Ca2+ in t...
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Article
Sensitive detection of micrometastases in bone marrow from patients with breast cancer using immunomagnetic isolation of tumor cells in combination with reverse transcriptase/polymerase chain reaction for cytokeratin-19
We report a highly sensitive method to detect rare human breast cancer cells, which combines an immunomagnetic separation (IMS) using antibody BM2 against MUC-1 with cytokeratin-19 (CK19) and the reverse tran...
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Metabolism of the hamster pancreatic carcinogen methyl-2-oxopropylnitrosamine by hamster liver and pancreas
Background. The mechanism whereby methyl-2-oxopropylnitrosamine (MOP) is activated remains unknown. To begin investigating this mechanism, we followed MOP disappearance during its incubation with...
