14 Result(s)

Article

Confinement in crystal lattice alters entire photocycle pathway of the Photoactive Yellow Protein

Femtosecond time-resolved crystallography (TRC) on proteins enables resolving the spatial structure of short-lived photocycle intermediates. An open question is whether confinement and lower hydration of the p...

Patrick E. Konold, Enis Arik, Jörn Weißenborn, Jos C. Arents… in Nature Communications (2020)

Article

Using osmotic stress to stabilize mannitol production in Synechocystis sp. PCC6803

Mannitol is a C(6) polyol that is used in the food and medical sector as a sweetener and antioxidant, respectively. The sustainable production of mannitol, especially via the direct conversion of CO₂ by photosynt...

Wenyang Wu, Wei Du, Ruth Perez Gallego, Klaas J. Hellingwerf… in Biotechnology for Biofuels (2020)

Article

Exploring the low photosynthetic efficiency of cyanobacteria in blue light using a mutant lacking phycobilisomes

The ubiquitous chlorophyll a (Chl a) pigment absorbs both blue and red light. Yet, in contrast to green algae and higher plants, most cyanobacteria have much lower photosynthetic rates in blue than in red light. ...

Veerle M. Luimstra, J. Merijn Schuurmans… in Photosynthesis Research (2019)

Article

Blue light reduces photosynthetic efficiency of cyanobacteria through an imbalance between photosystems I and II

Several studies have described that cyanobacteria use blue light less efficiently for photosynthesis than most eukaryotic phototrophs, but comprehensive studies of this phenomenon are lacking. Here, we study t...

Veerle M. Luimstra, J. Merijn Schuurmans, Antonie M. Verschoor… in Photosynthesis Research (2018)

Article

Spectrally decomposed dark-to-light transitions in Synechocystis sp. PCC 6803

Photosynthetic activity and respiration share the thylakoid membrane in cyanobacteria. We present a series of spectrally resolved fluorescence experiments where whole cells of the cyanobacterium Synechocystis sp....

Alonso M. Acuña, Pascal van Alphen, Filipe Branco dos Santos… in Photosynthesis Research (2018)

Article

Design, characterization and in vivo functioning of a light-dependent histidine protein kinase in the yeast Saccharomyces cerevisiae

Helical alignment of the α-helical linker of the LOV (light-oxygen-voltage) domain of YtvA from Bacillus subtilis with the α-helical linker of the histidine-protein kinase domain of the Sln1 kinase of the phospho...

Aleksandra Bury, Klaas J. Hellingwerf in AMB Express (2018)

Article

Alignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth

Microbial bioengineering has the potential to become a key contributor to the future development of human society by providing sustainable, novel, and cost-effective production pipelines. However, the sustaine...

Wei Du, Joeri A. Jongbloets, Coco van Boxtel… in Biotechnology for Biofuels (2018)

Article

Ethylene production with engineered Synechocystis sp PCC 6803 strains

Metabolic engineering and synthetic biology of cyanobacteria offer a promising sustainable alternative approach for fossil-based ethylene production, by using sunlight via oxygenic photosynthesis, to convert c...

Vinod Puthan Veetil, S. Andreas Angermayr, Klaas J. Hellingwerf in Microbial Cell Factories (2017)

Article

Genetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol

Erythritol is a polyol that is used in the food and beverage industry. Due to its non-caloric and non-cariogenic properties, the popularity of this sweetener is increasing. Large scale production of erythritol...

Aniek D. van der Woude, Ruth Perez Gallego, Angie Vreugdenhil… in Microbial Cell Factories (2016)

Article

Single-cell screening of photosynthetic growth and lactate production by cyanobacteria

Photosynthetic cyanobacteria are attractive for a range of biotechnological applications including biofuel production. However, due to slow growth, screening of mutant libraries using microtiter plates is not ...

Petter Hammar, S. Andreas Angermayr, Staffan L. Sjostrom… in Biotechnology for Biofuels (2015)

Article

Quantitative proteomics analysis of an ethanol- and a lactate-producing mutant strain of Synechocystis sp. PCC6803

This study aimed at exploring the molecular physiological consequences of a major redirection of carbon flow in so-called cyanobacterial cell factories: quantitative whole-cell proteomics analyses were carried...

Orawan Borirak, Leo J de Koning, Aniek D van der Woude… in Biotechnology for Biofuels (2015)

Article

Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803

Molecular engineering of the intermediary physiology of cyanobacteria has become important for the sustainable production of biofuels and commodity compounds from CO₂ and sunlight by “designer microbes.” The chem...

S Andreas Angermayr, Aniek D van der Woude, Danilo Correddu… in Biotechnology for Biofuels (2014)

Article

Algal Photosynthesis as the Primary Driver for a Sustainable Development in Energy, Feed, and Food Production

High oil prices and global warming that accompany the use of fossil fuels are an incentive to find alternative forms of energy supply. Photosynthetic biofuel production represents one of these since for this, ...

Ida G. Anemaet, Martijn Bekker, Klaas J. Hellingwerf in Marine Biotechnology (2010)

Article

Characterization and transcriptional regulation of the Synechocystis PCC 6803, petH gene, encoding ferredoxin-NADP⁺ oxidoreductase: involvement of a novel type of divergent operator

Jasper J. van Thor, Klaas J. Hellingwerf, Hans C.P. Matthijs in Plant Molecular Biology (1998)