19 Result(s)

Article

Tryptophan fluorescence as a reporter for structural changes in photoactive yellow protein elicited by photo-activation

Light-activation of photoactive yellow protein (PYP) is followed by a series of dynamical transitions in the structure of the protein. Tryptophan fluorescence is well-suited as a tool to study selected aspects...

Marijke Hospes, Johnny Hendriks… in Photochemical & Photobiological Sciences (2013)

Article

Is the photoactive yellow protein a UV-B/blue light photoreceptor?

UV light below 300 nm is shown to generate the first photocycle intermediate in the blue light photoreceptor Photoactive Yellow Protein. Fluorescence and ultrafast transient absorption measurements indicate tw...

Elizabeth C. Carroll, Marijke Hospes… in Photochemical & Photobiological Sciences (2011)

Chapter

Photoreceptor Proteins from Purple Bacteria

Purple bacteria contain representatives of four of the six main families of photoreceptor proteins: phytochromes, BLUF domain containing proteins, xanthopsins (i.e., photoactive yellow proteins), and phototrop...

Johnny Hendriks, Michael A. van der Horst, Toh Kee Chua… in The Purple Phototrophic Bacteria (2009)

Article

Binding, tuning and mechanical function of the 4-hydroxy-cinnamic acid chromophore in photoactive yellow protein

The bacterial photoreceptor protein photoactive yellow protein (PYP) covalently binds the chromophore 4-hydroxy coumaric acid, tuning (spectral) characteristics of this cofactor. Here, we study this binding an...

Michael A. van der Horst, Jos C. Arents… in Photochemical & Photobiological Sciences (2007)

Chapter

Light-induced behavioral responses (‘phototaxis’) in prokaryotes

Light-induced sensory responses are among the oldest scientific observations on bacterial behavior. Various types of response have been characterized physiologically in detail. However, the molecular basis of ...

Judith P. Armitage, Klaas J. Hellingwerf in Discoveries in Photosynthesis (2005)

Article

Chromophore composition of a heterologously expressed BLUF-domain

Upon heterologous expression of the BLUF (for: Blue-Light sensing Using Flavin) domain from AppA, a transcriptional anti-repressor from Rhodobacter sphaeroides, in Escherichia coli, photoactive holo-protein is fo...

Wouter Laan, Teresa Bednarz, Joachim Heberle… in Photochemical & Photobiological Sciences (2004)

Article

Early molecular events in the photoactive yellow protein: role of the chromophore photophysics

We report a comparative study of the isomerization reaction in native and denatured photoactive yellow protein (PYP) and in various chromophore analogues in their trans deprotonated form. The excited-state relaxa...

Pascale Changenet-Barret, Agathe Espagne… in Photochemical & Photobiological Sciences (2004)

Article

Tryptophan fluorescence monitors structural changes accompanying signalling state formation in the photocycle of photoactive yellow protein

Photoactive yellow protein, a small, water-soluble blue-light absorbing photoreceptor protein from Ectothiorhodospira (Halorhodospira) halophila has a structure with two hydrophobic cores, of which the main one h...

Thomas Gensch, Johnny Hendriks… in Photochemical & Photobiological Sciences (2004)

Article

Light-induced behavioral responses (`phototaxis') in prokaryotes

Light-induced sensory responses are among the oldest scientific observations on bacterial behavior. Various types of response have been characterized physiologically in detail. However, the molecular basis of ...

Judith P. Armitage, Klaas J. Hellingwerf in Photosynthesis Research (2003)

Article

The molecular basis of sensing and responding to light in microorganisms

Photon absorption in biological signal transfer is mediated by a limited number of photoreceptor families, each characterised by binding of chromophore from a particular class of chemical compounds. Most photo...

Klaas J. Hellingwerf in Antonie van Leeuwenhoek (2002)

Article

Current topics in signal transduction in bacteria

Among the signal transfer systems in bacteria two types predominate: two-component regulatory systems and quorum sensing systems. Both types of system can mediate signal transfer across the bacterial cell enve...

Klaas J. Hellingwerf, Wim C. Crielaard… in Antonie van Leeuwenhoek (1998)

Article

Characterization and transcriptional regulation of the Synechocystis PCC 6803 petH gene, encoding ferredoxin-NADP⁺ oxidoreductase: involvement of a novel type of divergent operator

The petH gene, encoding ferredoxin-NADP⁺ oxidoreductase (FNR), has been characterised in the unicellular cyanobacterium Synechocystis PCC 6803. Its product, FNR, was heterologously produced and functionally chara...

Jasper J. van Thor, Klaas J. Hellingwerf, Hans C.P. Matthijs in Plant Molecular Biology (1998)

Article

Regulation of the expression of thePseudomonas stutzeri recA gene

With the aid ofrecA-lacZ fusion strains, thein vivo regulation of thePseudomonas stutzeri recA gene has been studied. It is shown that expression of this gene can be induced with a variety of DNA damaging agents,...

Ben Vosman, Peter J. G. Rauch, Hans V. Westerhoff… in Antonie van Leeuwenhoek (1993)

Article

Molecular cloning and functional characterization of a recA analog from Pseudomonas stutzeri and construction of a P. stutzeri recA mutant

A recombinant plasmid carrying the Pseudomonas stutzeri recA gene was isolated by complementation of the Escherichia coli recA13 mutation. Subcloning experiments showed that the gene was located on a 1500 bp PvuI...

Ben Vosman, Klaas J. Hellingwerf in Antonie van Leeuwenhoek (1991)

Article

The role of the proton motive force and electron flow in light-driven solute transport in Rhodopseudomonas sphaeroides

Marieke G. L. Elferink, Klaas J. Hellingwerf, Wil N. Konings in Antonie van Leeuwenhoek (1984)