23 Result(s)
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Article
Open AccessUsing osmotic stress to stabilize mannitol production in Synechocystis sp. PCC6803
Mannitol is a C(6) polyol that is used in the food and medical sector as a sweetener and antioxidant, respectively. The sustainable production of mannitol, especially via the direct conversion of CO2 by photosynt...
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Article
Open AccessDesign, characterization and in vivo functioning of a light-dependent histidine protein kinase in the yeast Saccharomyces cerevisiae
Helical alignment of the α-helical linker of the LOV (light-oxygen-voltage) domain of YtvA from Bacillus subtilis with the α-helical linker of the histidine-protein kinase domain of the Sln1 kinase of the phospho...
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Article
Open AccessAlignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth
Microbial bioengineering has the potential to become a key contributor to the future development of human society by providing sustainable, novel, and cost-effective production pipelines. However, the sustaine...
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Chapter
Challenges in the Application of Synthetic Biology Toward Synthesis of Commodity Products by Cyanobacteria via “Direct Conversion”
Cyanobacterial direct conversion of CO2 to several commodity chemicals has been recognized as a potential contributor to support the much-needed sustainable development of human societies. However, the feasibilit...
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Article
Open AccessEthylene production with engineered Synechocystis sp PCC 6803 strains
Metabolic engineering and synthetic biology of cyanobacteria offer a promising sustainable alternative approach for fossil-based ethylene production, by using sunlight via oxygenic photosynthesis, to convert c...
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Article
Open AccessGenetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol
Erythritol is a polyol that is used in the food and beverage industry. Due to its non-caloric and non-cariogenic properties, the popularity of this sweetener is increasing. Large scale production of erythritol...
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Article
Open AccessSingle-cell screening of photosynthetic growth and lactate production by cyanobacteria
Photosynthetic cyanobacteria are attractive for a range of biotechnological applications including biofuel production. However, due to slow growth, screening of mutant libraries using microtiter plates is not ...
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Article
Open AccessQuantitative proteomics analysis of an ethanol- and a lactate-producing mutant strain of Synechocystis sp. PCC6803
This study aimed at exploring the molecular physiological consequences of a major redirection of carbon flow in so-called cyanobacterial cell factories: quantitative whole-cell proteomics analyses were carried...
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Article
Open AccessExploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803
Molecular engineering of the intermediary physiology of cyanobacteria has become important for the sustainable production of biofuels and commodity compounds from CO2 and sunlight by “designer microbes.” The chem...
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Article
Open AccessAlgal Photosynthesis as the Primary Driver for a Sustainable Development in Energy, Feed, and Food Production
High oil prices and global warming that accompany the use of fossil fuels are an incentive to find alternative forms of energy supply. Photosynthetic biofuel production represents one of these since for this, ...
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Chapter
Photoreceptor Proteins from Purple Bacteria
Purple bacteria contain representatives of four of the six main families of photoreceptor proteins: phytochromes, BLUF domain containing proteins, xanthopsins (i.e., photoactive yellow proteins), and phototrop...
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Chapter
Light-induced behavioral responses (‘phototaxis’) in prokaryotes
Light-induced sensory responses are among the oldest scientific observations on bacterial behavior. Various types of response have been characterized physiologically in detail. However, the molecular basis of ...
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Article
The molecular basis of sensing and responding to light in microorganisms
Photon absorption in biological signal transfer is mediated by a limited number of photoreceptor families, each characterised by binding of chromophore from a particular class of chemical compounds. Most photo...
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Article
Alkalispirillum mobile gen. nov., spec. nov., an alkaliphilic non-phototrophic member of the Ectothiorhodospiraceae
From cultures of the anoxygenic phototroph Halorhodospira halophila SL-1, an aerobic, gram-negative spirillum was isolated. This moderately halophilic, alkaliphilic bacterium was motile by means of a single pola...
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Article
Current topics in signal transduction in bacteria
Among the signal transfer systems in bacteria two types predominate: two-component regulatory systems and quorum sensing systems. Both types of system can mediate signal transfer across the bacterial cell enve...
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Article
Effect of nitrogen sources on the regulation of extracellular lipase production in Acinetobacter calcoaceticus strains
Extracellular lipase production and lipA expression were studied in A.calcoaceticus strains in relation to the nitrogen sources. Addition of casamino-acids or tryptone improved 2–3 times lipase yield as compared ...
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Article
Acinetobacter calcoaceticus liberates chromosomal DNA during induction of competence by cell lysis
A transformation assay was used to assay the amount of DNA present in the extracellular medium of a growing culture of Acinetobacter calcoaceticus. It was observed that small amounts of DNA were liberated during ...
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Article
Physiological regulation of competence induction for natural transformation in Acinetobacter calcoaceticus
Acinetobacter calcoaceticus induced competence for natural transformation maximally after dilution of a stationary culture into fresh medium. Competence was gradually lost during prolonged exponential growth and...
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Article
Increased stability of recombinant plasmids by Tn1000 insertion in chemostat cultures of recombinantEscherichia coli GT123
The stability of several pBR322-derived recombinant plasmids, carrying thethr operon fromEscherichia coli, was investigated in sulfur-limited chemostat cultures ofE. coli GT123. A marked increase in the segregati...
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Article
Regulation of the expression of thePseudomonas stutzeri recA gene
With the aid ofrecA-lacZ fusion strains, thein vivo regulation of thePseudomonas stutzeri recA gene has been studied. It is shown that expression of this gene can be induced with a variety of DNA damaging agents,...
