14 Result(s)
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Article
Open AccessUsing osmotic stress to stabilize mannitol production in Synechocystis sp. PCC6803
Mannitol is a C(6) polyol that is used in the food and medical sector as a sweetener and antioxidant, respectively. The sustainable production of mannitol, especially via the direct conversion of CO2 by photosynt...
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Open AccessDesign, characterization and in vivo functioning of a light-dependent histidine protein kinase in the yeast Saccharomyces cerevisiae
Helical alignment of the α-helical linker of the LOV (light-oxygen-voltage) domain of YtvA from Bacillus subtilis with the α-helical linker of the histidine-protein kinase domain of the Sln1 kinase of the phospho...
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Open AccessAlignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth
Microbial bioengineering has the potential to become a key contributor to the future development of human society by providing sustainable, novel, and cost-effective production pipelines. However, the sustaine...
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Article
Open AccessEthylene production with engineered Synechocystis sp PCC 6803 strains
Metabolic engineering and synthetic biology of cyanobacteria offer a promising sustainable alternative approach for fossil-based ethylene production, by using sunlight via oxygenic photosynthesis, to convert c...
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Open AccessGenetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol
Erythritol is a polyol that is used in the food and beverage industry. Due to its non-caloric and non-cariogenic properties, the popularity of this sweetener is increasing. Large scale production of erythritol...
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Article
Open AccessSingle-cell screening of photosynthetic growth and lactate production by cyanobacteria
Photosynthetic cyanobacteria are attractive for a range of biotechnological applications including biofuel production. However, due to slow growth, screening of mutant libraries using microtiter plates is not ...
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Open AccessQuantitative proteomics analysis of an ethanol- and a lactate-producing mutant strain of Synechocystis sp. PCC6803
This study aimed at exploring the molecular physiological consequences of a major redirection of carbon flow in so-called cyanobacterial cell factories: quantitative whole-cell proteomics analyses were carried...
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Article
Open AccessExploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803
Molecular engineering of the intermediary physiology of cyanobacteria has become important for the sustainable production of biofuels and commodity compounds from CO2 and sunlight by “designer microbes.” The chem...
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Article
Alkalispirillum mobile gen. nov., spec. nov., an alkaliphilic non-phototrophic member of the Ectothiorhodospiraceae
From cultures of the anoxygenic phototroph Halorhodospira halophila SL-1, an aerobic, gram-negative spirillum was isolated. This moderately halophilic, alkaliphilic bacterium was motile by means of a single pola...
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Article
Effect of nitrogen sources on the regulation of extracellular lipase production in Acinetobacter calcoaceticus strains
Extracellular lipase production and lipA expression were studied in A.calcoaceticus strains in relation to the nitrogen sources. Addition of casamino-acids or tryptone improved 2–3 times lipase yield as compared ...
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Article
Acinetobacter calcoaceticus liberates chromosomal DNA during induction of competence by cell lysis
A transformation assay was used to assay the amount of DNA present in the extracellular medium of a growing culture of Acinetobacter calcoaceticus. It was observed that small amounts of DNA were liberated during ...
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Article
Physiological regulation of competence induction for natural transformation in Acinetobacter calcoaceticus
Acinetobacter calcoaceticus induced competence for natural transformation maximally after dilution of a stationary culture into fresh medium. Competence was gradually lost during prolonged exponential growth and...
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Article
Increased stability of recombinant plasmids by Tn1000 insertion in chemostat cultures of recombinantEscherichia coli GT123
The stability of several pBR322-derived recombinant plasmids, carrying thethr operon fromEscherichia coli, was investigated in sulfur-limited chemostat cultures ofE. coli GT123. A marked increase in the segregati...
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Article
A Kdp-like, high-affinity, K+-translocating ATPase is expressed during growth of Rhodobacter sphaeroides in low potassium media
Cells of the purple non-sulphur bacterium Rhodobacter sphaeroides express a high-affinity K+ uptake system when grown in media with low K+ concentrations. Antibodies againts the catalytic KdpB protein or the whol...
