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Protocol
Prokaryotic Phototaxis
Microorganisms have various mechanisms at their disposal to react to (changes in) their ambient light climate (i.e., intensity, color, direction, and degree of polarization). Of these, one of the best studied ...
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Chapter
Light-induced behavioral responses (‘phototaxis’) in prokaryotes
Light-induced sensory responses are among the oldest scientific observations on bacterial behavior. Various types of response have been characterized physiologically in detail. However, the molecular basis of ...
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Protocol
8 Fluorescence Resonance Energy Transfer (FRET) Applications Using Green Fluorescent Protein
Fluorescence resonance energy transfer (FRET) is a technique that can be used to estimate intermolecular distances between pigment molecules, which is an approach first proposed by Stryer (1). The theoretical bas...
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Article
Alkalispirillum mobile gen. nov., spec. nov., an alkaliphilic non-phototrophic member of the Ectothiorhodospiraceae
From cultures of the anoxygenic phototroph Halorhodospira halophila SL-1, an aerobic, gram-negative spirillum was isolated. This moderately halophilic, alkaliphilic bacterium was motile by means of a single pola...
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Article
Physiological regulation of competence induction for natural transformation in Acinetobacter calcoaceticus
Acinetobacter calcoaceticus induced competence for natural transformation maximally after dilution of a stationary culture into fresh medium. Competence was gradually lost during prolonged exponential growth and...
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Article
A Kdp-like, high-affinity, K+-translocating ATPase is expressed during growth of Rhodobacter sphaeroides in low potassium media
Cells of the purple non-sulphur bacterium Rhodobacter sphaeroides express a high-affinity K+ uptake system when grown in media with low K+ concentrations. Antibodies againts the catalytic KdpB protein or the whol...
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Chapter and Conference Paper
Functional Reconstitution of Photosynthetic Reaction Centre Complexes from Rhodopseudomonas Palustris
Reconstitution is a powerful technique in the elucidation of the mechanisms involved in membrane associated biological energy transduction (see for example Racker & Stoeckenius, 1974). For proton motive force ...