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    Protocol

    Prokaryotic Phototaxis

    Microorganisms have various mechanisms at their disposal to react to (changes in) their ambient light climate (i.e., intensity, color, direction, and degree of polarization). Of these, one of the best studied ...

    Wouter D. Hoff, Michael A. van der Horst, Clara B. Nudel in Chemotaxis (2009)

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    Chapter

    Light-induced behavioral responses (‘phototaxis’) in prokaryotes

    Light-induced sensory responses are among the oldest scientific observations on bacterial behavior. Various types of response have been characterized physiologically in detail. However, the molecular basis of ...

    Judith P. Armitage, Klaas J. Hellingwerf in Discoveries in Photosynthesis (2005)

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    Protocol

    8 Fluorescence Resonance Energy Transfer (FRET) Applications Using Green Fluorescent Protein

    Fluorescence resonance energy transfer (FRET) is a technique that can be used to estimate intermolecular distances between pigment molecules, which is an approach first proposed by Stryer (1). The theoretical bas...

    Jasper van J. Thor, Klaas J. Hellingwerf in Green Fluorescent Protein (2002)

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    Article

    Alkalispirillum mobile gen. nov., spec. nov., an alkaliphilic non-phototrophic member of the Ectothiorhodospiraceae

    From cultures of the anoxygenic phototroph Halorhodospira halophila SL-1, an aerobic, gram-negative spirillum was isolated. This moderately halophilic, alkaliphilic bacterium was motile by means of a single pola...

    Micha J. Rijkenberg, Remco Kort, Klaas J. Hellingwerf in Archives of Microbiology (2001)

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    Article

    Physiological regulation of competence induction for natural transformation in Acinetobacter calcoaceticus

    Acinetobacter calcoaceticus induced competence for natural transformation maximally after dilution of a stationary culture into fresh medium. Competence was gradually lost during prolonged exponential growth and...

    Ronald Palmen, Pieter Buijsman, Klaas J. Hellingwerf in Archives of Microbiology (1994)

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    Article

    A Kdp-like, high-affinity, K+-translocating ATPase is expressed during growth of Rhodobacter sphaeroides in low potassium media

    Cells of the purple non-sulphur bacterium Rhodobacter sphaeroides express a high-affinity K+ uptake system when grown in media with low K+ concentrations. Antibodies againts the catalytic KdpB protein or the whol...

    Tjakko Abee, Jan Knol, Klaas J. Hellingwerf, Evert P. Bakker in Archives of Microbiology (1992)

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    Chapter and Conference Paper

    Functional Reconstitution of Photosynthetic Reaction Centre Complexes from Rhodopseudomonas Palustris

    Reconstitution is a powerful technique in the elucidation of the mechanisms involved in membrane associated biological energy transduction (see for example Racker & Stoeckenius, 1974). For proton motive force ...

    Douwe Molenaar, Wim Crielaard in Receptors, Membrane Transport and Signal T… (1989)