38 Result(s)
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Protocol
Development of a Light-Dependent Protein Histidine Kinase
Phosphorylation plays a critical role in facilitating signal transduction in prokaryotic and eukaryotic organisms. Our study introduces a tool for investigation of signal diffusion in a biochemical regulation ...
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Article
Open AccessExploring the low photosynthetic efficiency of cyanobacteria in blue light using a mutant lacking phycobilisomes
The ubiquitous chlorophyll a (Chl a) pigment absorbs both blue and red light. Yet, in contrast to green algae and higher plants, most cyanobacteria have much lower photosynthetic rates in blue than in red light. ...
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Article
Open AccessBlue light reduces photosynthetic efficiency of cyanobacteria through an imbalance between photosystems I and II
Several studies have described that cyanobacteria use blue light less efficiently for photosynthesis than most eukaryotic phototrophs, but comprehensive studies of this phenomenon are lacking. Here, we study t...
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Article
Open AccessSpectrally decomposed dark-to-light transitions in Synechocystis sp. PCC 6803
Photosynthetic activity and respiration share the thylakoid membrane in cyanobacteria. We present a series of spectrally resolved fluorescence experiments where whole cells of the cyanobacterium Synechocystis sp....
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Article
Open AccessDesign, characterization and in vivo functioning of a light-dependent histidine protein kinase in the yeast Saccharomyces cerevisiae
Helical alignment of the α-helical linker of the LOV (light-oxygen-voltage) domain of YtvA from Bacillus subtilis with the α-helical linker of the histidine-protein kinase domain of the Sln1 kinase of the phospho...
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Protocol
On the In Vivo Redox State of Flavin-Containing Photosensory Receptor Proteins
Measured values of the redox midpoint potential of flavin-containing photoreceptor proteins range from physiologically very negative values, i.e., < –300 mV (compared to the calomel electrode) for some LOV dom...
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Article
Open AccessAlgal Photosynthesis as the Primary Driver for a Sustainable Development in Energy, Feed, and Food Production
High oil prices and global warming that accompany the use of fossil fuels are an incentive to find alternative forms of energy supply. Photosynthetic biofuel production represents one of these since for this, ...
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Chapter
Photoreceptor Proteins from Purple Bacteria
Purple bacteria contain representatives of four of the six main families of photoreceptor proteins: phytochromes, BLUF domain containing proteins, xanthopsins (i.e., photoactive yellow proteins), and phototrop...
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Protocol
Prokaryotic Phototaxis
Microorganisms have various mechanisms at their disposal to react to (changes in) their ambient light climate (i.e., intensity, color, direction, and degree of polarization). Of these, one of the best studied ...
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Chapter
Light-induced behavioral responses (‘phototaxis’) in prokaryotes
Light-induced sensory responses are among the oldest scientific observations on bacterial behavior. Various types of response have been characterized physiologically in detail. However, the molecular basis of ...
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Article
Light-induced behavioral responses (`phototaxis') in prokaryotes
Light-induced sensory responses are among the oldest scientific observations on bacterial behavior. Various types of response have been characterized physiologically in detail. However, the molecular basis of ...
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Article
The molecular basis of sensing and responding to light in microorganisms
Photon absorption in biological signal transfer is mediated by a limited number of photoreceptor families, each characterised by binding of chromophore from a particular class of chemical compounds. Most photo...
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Article
The Metabolic Response of Saccharomyces Cerevisiae to Continuous Heat Stress
A study has been initiated to integrate molecular and physiological responses of Saccharomyces cerevisiae to heat stress conditions. We focus our research on a quantification of the energetics of the stress respo...
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Article
Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222
Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP398) into one absorbing at 483 n...
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Protocol
8 Fluorescence Resonance Energy Transfer (FRET) Applications Using Green Fluorescent Protein
Fluorescence resonance energy transfer (FRET) is a technique that can be used to estimate intermolecular distances between pigment molecules, which is an approach first proposed by Stryer (1). The theoretical bas...
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Article
Alkalispirillum mobile gen. nov., spec. nov., an alkaliphilic non-phototrophic member of the Ectothiorhodospiraceae
From cultures of the anoxygenic phototroph Halorhodospira halophila SL-1, an aerobic, gram-negative spirillum was isolated. This moderately halophilic, alkaliphilic bacterium was motile by means of a single pola...
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Chapter
Sensing of Green Light in Complementary Chromatic Adaptation of the Cyanobacterium Calothrix sp.
Light harvesting is essential for phototrophic organisms. The reaction centers of PS2 and PS1 in cyanobacteria contain chl a, † a ubiquitous pigment that absorbs light in two spectral domains. In the blue, absorp...
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Article
Current topics in signal transduction in bacteria
Among the signal transfer systems in bacteria two types predominate: two-component regulatory systems and quorum sensing systems. Both types of system can mediate signal transfer across the bacterial cell enve...
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Article
Characterization and transcriptional regulation of the Synechocystis PCC 6803, petH gene, encoding ferredoxin-NADP+ oxidoreductase: involvement of a novel type of divergent operator
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Article
Structural and dynamic changes of photoactive yellow protein during its photocycle in solution
Light irradiation of photoactive yellow protein (PYP) induces a photocycle, in which red-shifted (pR) and blue-shifted (pB) intermediates have been characterized. An NMR study of the long-lived pB intermediate...
