17 Result(s)
within
Klaas J. Hellingwerf
Chemistry
English
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Article
Open AccessUsing osmotic stress to stabilize mannitol production in Synechocystis sp. PCC6803
Mannitol is a C(6) polyol that is used in the food and medical sector as a sweetener and antioxidant, respectively. The sustainable production of mannitol, especially via the direct conversion of CO2 by photosynt...
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Article
Open AccessAlignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth
Microbial bioengineering has the potential to become a key contributor to the future development of human society by providing sustainable, novel, and cost-effective production pipelines. However, the sustaine...
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Article
Open AccessEthylene production with engineered Synechocystis sp PCC 6803 strains
Metabolic engineering and synthetic biology of cyanobacteria offer a promising sustainable alternative approach for fossil-based ethylene production, by using sunlight via oxygenic photosynthesis, to convert c...
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Open AccessGenetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol
Erythritol is a polyol that is used in the food and beverage industry. Due to its non-caloric and non-cariogenic properties, the popularity of this sweetener is increasing. Large scale production of erythritol...
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Article
Open AccessSingle-cell screening of photosynthetic growth and lactate production by cyanobacteria
Photosynthetic cyanobacteria are attractive for a range of biotechnological applications including biofuel production. However, due to slow growth, screening of mutant libraries using microtiter plates is not ...
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Article
Open AccessQuantitative proteomics analysis of an ethanol- and a lactate-producing mutant strain of Synechocystis sp. PCC6803
This study aimed at exploring the molecular physiological consequences of a major redirection of carbon flow in so-called cyanobacterial cell factories: quantitative whole-cell proteomics analyses were carried...
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Open AccessExploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803
Molecular engineering of the intermediary physiology of cyanobacteria has become important for the sustainable production of biofuels and commodity compounds from CO2 and sunlight by “designer microbes.” The chem...
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Article
Tryptophan fluorescence as a reporter for structural changes in photoactive yellow protein elicited by photo-activation
Light-activation of photoactive yellow protein (PYP) is followed by a series of dynamical transitions in the structure of the protein. Tryptophan fluorescence is well-suited as a tool to study selected aspects...
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Article
Is the photoactive yellow protein a UV-B/blue light photoreceptor?
UV light below 300 nm is shown to generate the first photocycle intermediate in the blue light photoreceptor Photoactive Yellow Protein. Fluorescence and ultrafast transient absorption measurements indicate tw...
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Article
Binding, tuning and mechanical function of the 4-hydroxy-cinnamic acid chromophore in photoactive yellow protein
The bacterial photoreceptor protein photoactive yellow protein (PYP) covalently binds the chromophore 4-hydroxy coumaric acid, tuning (spectral) characteristics of this cofactor. Here, we study this binding an...
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Article
Chromophore composition of a heterologously expressed BLUF-domain
Upon heterologous expression of the BLUF (for: Blue-Light sensing Using Flavin) domain from AppA, a transcriptional anti-repressor from Rhodobacter sphaeroides, in Escherichia coli, photoactive holo-protein is fo...
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Article
Early molecular events in the photoactive yellow protein: role of the chromophore photophysics
We report a comparative study of the isomerization reaction in native and denatured photoactive yellow protein (PYP) and in various chromophore analogues in their trans deprotonated form. The excited-state relaxa...
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Article
Tryptophan fluorescence monitors structural changes accompanying signalling state formation in the photocycle of photoactive yellow protein
Photoactive yellow protein, a small, water-soluble blue-light absorbing photoreceptor protein from Ectothiorhodospira (Halorhodospira) halophila has a structure with two hydrophobic cores, of which the main one h...
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Chapter
Global bioenergetics
The surface of the earth is subject to a continuous input of free enthalpy originating in the sun. A small, but important part of this free enthalpy is diverted, transiently, to physical chemical structures th...
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Article
Reaction centers fromRhodopseudomonas sphaeroides in reconstituted phospholipid vesicles. II. Light-induced proton translocation
Unidirectional light-dependent proton translocation was demonstrated in a suspension of reconstituted reaction center (RC) vesicles supplemented with cytochromec and 2,3-dimethoxy-5-methyl-1,4-benzoquinone (UQ0),...
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Article
Reaction centers fromRhodopseudomonas sphaeroides in reconstituted phospholipid vesicles. I. Structural studies
Reaction centers (RCs) fromRhodopseudomonas sphaeroides were reconstituted into asolectin vesicles by cosonication. Equilibrium centrifugation on sucrose gradients showed that the vesicles were homogeneous in den...
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Article
The protein composition of the cytoplasmic membrane of aerobically and anaerobically grownEscherichia coli
The protein composition of the cytoplasmic membranes ofEscherichia coli, grown aerobically and anaerobically on a glucose minimal medium at pH 7.0, were analyzed by crossed immunoelectrophoresis. Qualitative diff...
