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Article
Preliminary survey of taxonomical problems, pharmacognostical characteristics, and chloroplast DNA polymorphisms of the folk medicinal herb Artemisia campestris from the Ryukyu Islands, Japan
Artemisia campestris L. (Compositae) occurs naturally along the coastline of the Ryukyu Islands and has been traditionally used as a folk medicine for the treatment of liver and kidney disorders. The authors obta...
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Article
Genetic transformation of gentian using wild-type Agrobacterium rhizogenes
Agrobacterium rhizogenes A4 harboring the agropine type root-inducing plasmid (pRiA4) induced hairy roots on stem sections of gentian plants. Transgenic gential plants were regenerated from the hairy roots at ...
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Article
Adventitious shoot regeneration from leaf, stem and root explants of commercial cultivars of Gentiana
Several culture conditions were examined for promoting efficient plant regeneration from explants of Gentiana. Adventitious shoot regeneration from leaf explants of cv. WSP-3 was very superior on MS medium, compa...
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Article
Production of essential oils by flowers of Hyacinthus orientalis L. regenerated in vitro
Essential oils were produced by flowers of Hyacinthus orientalis L. that had been regenerated in vitro. The production of these oils was affected by the concentration of gibberellic acid and sucrose in the medium...
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Article
Plant regeneration from protoplasts of Gentiana by embedding protoplasts in gellan gum
A protoplast-to-plant system was developed in Gentiana using a gellan gum-embedding culture. Viable protoplasts could be routinely isolated from in vitro-grown plantlets, and they were embedded in 0.2% gellan gum...