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    Article

    Activation of a Refolded, Berberine-specific, Single-chain Fv Fragment by Addition of Free Berberine

    A single-chain variable fragment (scFv) specific for berberine was produced in Escherichia coli. The anti-berberine scFv gene was cloned from hybridoma 1D5-3B-7 producing the monoclonal antibody. The variable reg...

    Jun-Sik Kim, Tadashi Masaki, Supaart Sirikantaramas… in Biotechnology Letters (2006)

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    Article

    Development of monoclonal antibody against isoquinoline alkaloid coptisine and its application for the screening of medicinal plants

    In the development of immunoassay technique, the design of hapten containing a functional group suitable for protein conjugate is the key step for the preparation of antibodies against small molecules. Coptisi...

    Jun-Sik Kim, Hiroyuki Tanaka, Chun-Su Yuan, Yukihiro Shoyama in Cytotechnology (2004)