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    Activation of a Refolded, Berberine-specific, Single-chain Fv Fragment by Addition of Free Berberine

    A single-chain variable fragment (scFv) specific for berberine was produced in Escherichia coli. The anti-berberine scFv gene was cloned from hybridoma 1D5-3B-7 producing the monoclonal antibody. The variable reg...

    Jun-Sik Kim, Tadashi Masaki, Supaart Sirikantaramas… in Biotechnology Letters (2006)