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    A PCR-based high-throughput screen with multiround sample pooling: application to somatic cell gene targeting

    Here, we describe a method of systematic PCR screening with multiround sample pooling for the isolation of rare PCR-positive samples. As an example, we have applied this protocol to the recovery of gene-target...

    Hiroyuki Konishi, Josh Lauring, Joseph P Garay, Bedri Karakas… in Nature Protocols (2007)