-
Article
Stimulation of artemisinin production in Artemisia annua hairy roots by the elicitor from the endophytic Colletotrichum sp.
Artemisinin content in hairy roots of Artemisia annua was increased from 0.8 mg g−1 dry wt to 1 mg g−1 dry wt by using elicitor treatment of mycelial extracts from the endophytic fungus Colletotrichum sp. The inc...
-
Article
Artemisinin production in Artemisia annua hairy root cultures with improved growth by altering the nitrogen source in the medium
Murashige & Skoog medium was modified for enhancing artemisinin production in Artemisia annua hairy root cultures by altering the ratio of NO 3 − /NH ...
-
Article
Improved artemisinin accumulation in hairy root cultures of Artemisia annua by (22S, 23S)-homobrassinolide
When (22S, 23S)-homobrassinolide (SSHB) was added at 1 μg l−1 to hairy root cultures of Artemisia annua, the production of artemisinin reached to 14 mg l−1, an increment of 57% over the control. SSHB treatment le...
-
Article
Preparation and characterization of pH sensitive sugar mediated (polyethylene glycol/chitosan) membrane
Novel biodegradable membrane based on chitosan matrix was prepared and characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and swelling test. Native sugar, which i...
-
Article
Biodegradation behavior and cytotoxicity of the composite membrane composed of β-dicalcium pyrophosphate and glucose mediated (polyethylene glycol/chitosan)
The purpose of this study is to prepare and evaluate the biodegradation behavior and cytotoxicity of a composite membrane, G-β-DCP, combining β-dicalcium pyrophosphate (β-DCP) ceramic particles and glucose med...
-
Article
High-dose chemotherapy with autologous peripheral blood stem cell support in children with malignant diseases
Objective: To determine the potential effectiveness and toxicity of this therapy in children with advanced neuroblastoma or malignant lymphoma. Methods: Carboplatin (425 mg/m2·d) and etoposide (3...
-
Article
Estimation of vegetation biophysical parameters by remote sensing using radial basis function neural network
Hyperspectral reflectance (350∼2500 nm) data were recorded at two different sites of rice in two experiment fields including two cultivars, and three levels of nitrogen (N) application. Twenty-five Vegetation ...
-
Article
Involvement of nitric oxide in cerebroside-induced defense responses and taxol production in Taxus yunnanensis suspension cells
This work was to characterize the generation of nitric oxide (NO) in Taxus yunnanensis cells induced by a fungal-derived cerebroside and the signal role of NO in the elicitation of plant defense responses and tax...
-
Article
Numerical simulation on effect of pressure distribution of wind turbine blade with a tip vane
It is important to study the pressure distribution on the blade and in the adjacent area while searching the power augmentation theory with adding a tip vane to the wind turbine. This paper shows the CFD simul...
-
Article
Stimulation of artemisinin synthesis by combined cerebroside and nitric oxide elicitation in Artemisia annua hairy roots
This work examined the accumulation of artemisinin and related secondary metabolism pathways in hairy root cultures of Artemisia annua L. induced by a fungal-derived cerebroside (2S,2′R,3R,3′E,4E,8E)-1-O-β-d-gluc...
-
Article
Tanshinone biosynthesis in Salvia miltiorrhiza and production in plant tissue cultures
Salvia miltiorrhiza Bunge (Lamiaceae) root, generally called Danshen, is an important herb in Chinese medicine widely used for treatment of cardiovascular diseases. Diterpenoid tanshinons are major bioactive cons...
-
Article
Nitric oxide elicitation for secondary metabolite production in cultured plant cells
Nitric oxide (NO) is an important signal molecule in stress responses. Accumulation of secondary metabolites often occurs in plants subjected to stresses including various elicitors or signal molecules. NO has...
-
Chapter
Effective Elicitors and Process Strategies for Enhancement of Secondary Metabolite Production in Hairy Root Cultures
This chapter reviews the various biotic and abiotic elicitors applied to hairy root cultures and their stimulating effects on the accumulation of secondary metabolites. Elicitors generally refer to the agents ...
-
Article
Retroperitoneal laparoendoscopic single-site surgery for the treatment of retrocaval ureter
To present our surgical techniques for retroperitoneal laparoendoscopic single-site (LESS) pyelopyelostomy for retrocaval ureter and our initial experience with this method in 4 patients.
-
Article
Epidemiological and clinical characteristics of 266 cases of intracerebral hemorrhage in Hangzhou, China
Ethnicity and socioeconomic factors can influence disease susceptibility, clinical presentation, and outcome. We investigated the clinical characteristics (age, sex, seasonal variation, lesion site, symptoms, ...
-
Article
Glaucocalyxin A and B Regulate Growth and Induce Oxidative Stress in Lettuce (Lactuca sativa L.) Roots
Glaucocalyxin (Gla) A–C are major ent-kaurane diterpenoids isolated from Isodon japonicus var. glaucocalyx (Maxim.) H. W. Li. This study investigated the possible interference of these diterpenoids with root grow...
-
Article
Time resolved particle image velocimetry experimental study of the near wake characteristics of a horizontal axis wind turbine
Wind tunnel experiments at a model scale have been carried out to investigate the flow characteristics in the near wake of a wind turbine. Time resolved particle image velocimetry (TRPIV) measurements are appl...
-
Article
PEGylation of cytochrome c at the level of lysine residues mediated by a microbial transglutaminase
To establish a method for microbial transglutaminase (mTG)-mediated PEGylation of proteins at the level of lysine (Lys) residues.
-
Article
The influence of endophytic Penicillium oxalicum B4 on growth and artemisinin biosynthesis of in vitro propagated plantlets of Artemisia annua L.
Artemisia annua L. is presently the sole natural source of antimalarial drug artemisinin. We established dual cultures of A. annua callus or regenerated plantlets with endophytic Penicill...
-
Article
Multienzymatic cascade synthesis of fucosyloligosaccharide via a two-step fermentation strategy in Escherichia coli
To achieve multienzymatic cascade synthesis of fucosyl oligosaccharide from d-mannose by two-step fermentation pathway in Escherichia coli.
