7 Result(s)
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Chapter
Nucleolar Organizer Regions as Transcription-Based Scaffolds of Nucleolar Structure and Function
Eukaryotic genomes maintain multiple copies of ribosomal DNA gene repeats in tandem arrays to provide sufficient ribosomal RNAs to make ribosomes. These DNA repeats are the most highly transcribed regions of t...
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Article
Ribosomal DNA-connecting ribosome biogenesis and chromosome biology
Ribosomal DNA, the topic of this special issue, has long fascinated biologists. The RNA products of the ribosomal DNA are the ribosomal RNAs that are part of the ribosome. In this special issue, we focus on th...
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Article
Ribosomal DNA and the nucleolus in the context of genome organization
The nucleolus constitutes a prominent nuclear compartment, a membraneless organelle that was first documented in the 1830s. The fact that specific chromosomal regions were present in the nucleolus was recogniz...
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Article
Ribosomal DNA instability and genome adaptability
Ribosomes are large, multi-subunit ribonucleoprotein complexes, essential for protein synthesis. To meet the high cellular demand for ribosomes, all eukaryotes have numerous copies of ribosomal DNA (rDNA) gene...
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Article
Regulation of kinetochore configuration during mitosis
Successful proliferation and function of an organism relies on the equal segregation of its genetic material during cell division. Duplicate sister chromatids need to accurately segregate at mitosis. Precise s...
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Protocol
Using Fluorescent Reporters in Conjunction with Cytometry and Statistics to Assess Nuclear Accumulation of Ribosomal Proteins
Fluorescently labeled ribosomal proteins can be used to detect and monitor the intracellular localization of these proteins. Both Rps2, a subunit of the 40S ribosome, and Rpl25, a subunit of the 60S ribosome, ...
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Article
Open AccessIntersection of ChIP and FLIP, genomic methods to study the dynamics of the cohesin proteins
The evolutionarily conserved cohesin proteins Smc1, Smc3, Rad21 (Mcd1), and Scc3 function in the cohesin complex that provides the basis for chromosome cohesion and is involved in gene regulation. Understandin...
