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Article
An integrated strategy for the process development of a recombinant antibody-cytokine fusion protein expressed in BHK cells
Recombinant fusion proteins offer important new therapeutic approaches for the future. This report describes the use of three different genetic strategies (i.e. “mono-”, “bi-” and “tri-cistronic” vectors) to ...
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Chapter
Alternative Strategies for the Production of an Antibody-Cytokine Fusion Protein from Recombinant BHK Cells
The choice of scale up strategies for production of recombinant proteins from animal cells is often restricted to suspension cultures in stirred tank bioreactors. The primary aim of the work reported here was ...
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Book
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Chapter
Evaluation of a Porous Silicone Carrier (Immobasil G) for Animal Cell Culture
Porous carriers can be used in fixed, fluidised and stirred tank perfusion culture systems, where high unit cell density and good scale-up potential can be combined with longterm continuous operation (1 and 2)...
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Chapter
Cultural and physiological factors affecting expression of recombinant proteins
The variability in expression of recombinant proteins has been analyzed with regard to (a) comparison of clones from the same transfection experiment; (b) comparison of the same genetic construct in different ...
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Article
Expression of recombinant antibody and secreted alkaline phosphatase in mammalian cells. Influence of cell line and culture system upon production kinetics
The growth and productivity of an Sp2/0 cell line, F3b10, expressing a recombinant antibody (rAb) and BHK21 cells expressing either the same rAb from the same plasmids (BHK.IgG) or secreted alkaline phosphatas...
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Chapter
Recombinant Antibody Expression in a Fixed Bed Perfusion System
Process parameters affecting the productivity of a fixed bed bioreactor (FBR) system, based on Siran beads, have been studied. A recombinant BHK21 cell line was grown with different combinations of process par...