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Article
Proteolytic stability of recombinant human serum albumin secreted in the yeast Saccharomyces cerevisiae
In order to direct the persistent expression of recombinant human serum albumin (HSA) from the GAL10 promoter in the yeast Saccharomyces cerevisiae, we carried out periodic feeding of galactose during shake-flas...
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Article
Molecular characterization of a cDNA encoding putative calcium binding protein, HvCaBP1, induced during kernel development in barley (Hordeum vulgare L.)
Seed development is known to involve complex physiological and molecular events. In order to gain information on the molecular events that occur in the grains of barley during kernel development, we conducted ...
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Article
Expressed sequence tags from a wheat-rye translocation line (2BS/2RL) infested by larvae of Hessian fly [Mayetiola destructor (Say)]
Of the 16 known biotypes of the Hessian fly [Mayetiola destructor (Say)], biotype L is recognized as being the most virulent. We have previously reported the development of near-isogenic lines (NILs) (BC3F3:4) by...
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Article
A new selection method for pepper transformation: callus-mediated shoot formation
We used two genes, TMV-CP and PPI1 (pepper-PMMV interaction 1 transcription factor), to transform commercially important chili pepper (Capsicum annuum) inbred lines (P915, P409) by means of Agrobacterium co-cultu...
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Article
AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants
Calmodulin (CaM) influences many cellular processes by interacting with various proteins. Here, we isolated AtBAG6, an Arabidopsis CaM-binding protein that contains a central BCL-2-associated athanogene (BAG) dom...
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Article
Expressed sequence tags from wheat roots under hypoxia
Hypoxia due to waterlogging adversely affects agricultural crops in many parts of the world and severely impacts overall annual production levels. To evaluate the response of wheat roots to a controlled hypoxi...
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Article
The putative tumor suppressor gene GLTSCR2 induces PTEN-modulated cell death
Glioma tumor suppressor candidate region gene 2 (GLTSCR2/PICT-1) is localized within the well-known 1.4-Mb tumor suppressive region of chromosome 19q, which is frequently altered in various human tumors, inclu...
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Article
Molecular characterization of a cDNA encoding disulfide isomerase during barley kernel development
We conducted differential hybridization using two different tissues (caryopses as a tester and pericarps as a driver) 14 days after fertilization (DAF) to characterize the molecular mechanisms inherent to barl...
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Article
Discovery of SNPs in the swine nerve growth factor gene
This study was aimed to search genetic variants for the swine nerve growth factor gene that associated with regulation of proliferation and differentiation of nervous systems. The swine nerve growth factor gen...
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Article
Open AccessSoluble intracellular adhesion molecule-1 secreted by human umbilical cord blood-derived mesenchymal stem cell reduces amyloid-β plaques
Presently, co-culture of human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) with BV2 microglia under amyloid-β42 (Aβ42) exposure induced a reduction of Aβ42 in the medium as well as an overexpression o...
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Article
Nucleolar protein GLTSCR2 stabilizes p53 in response to ribosomal stresses
p53 is a key regulator of cell growth and death by controlling cell cycle progression and apoptosis under conditions of stress such as DNA damage or oncogenic stimulation. As these processes are critical for c...
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Article
Functional characterization of Arabidopsis Dof transcription factor AtDof4.1
The plant specific Dof (DNA Binding with One Zinc Finger) transcription factors (TFs) perform many different functions, and some members of this family appear to play an important role in the evolution of the ...
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Article
Recovery from Parkinsonism with N-acetylcysteine-differentiated neurons
The upregulation of dopaminergic neuronal differentiation is necessary for stem cell therapy in Parkinson’s disease (PD). In this study, neuronal differentiation efficiency increased by more than 2 times in P1...
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Article
Open AccessMicroRNA-302 induces proliferation and inhibits oxidant-induced cell death in human adipose tissue-derived mesenchymal stem cells
Mesenchymal stem cells (MSCs) are a heterogeneous population of cells that proliferate in vitro as plastic-adherent cells, have a fibroblast-like morphology, form colonies in vitro and can differentiate into bone...
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Article
Open AccessPARP1 activation/expression modulates regional-specific neuronal and glial responses to seizure in a hemodynamic-independent manner
Poly(ADP-ribose) polymerase-1 (PARP1) plays a regulatory role in apoptosis, necrosis and other cellular processes after injury. Status epilepticus (SE) induces neuronal and astroglial death that show regional-...
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Article
Early life stress experience may blunt hypothalamic leptin signalling
The aim of this study was to investigate whether neonatal maternal separation (MS) – chronic stress experience in early life – affects the anorectic efficacy of leptin in the offspring at adolescence. Sprague–...
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Article
Generation of Transgenic Napier Grass (Cenchrus purpureus Schum.) Plants by Biolistic Gene Transfer of a Minimal Expression Cassette
Napier grass (Cenchrus purpureus Schum.) is one of the highest yielding tropical C4 forage grasses and a promising candidate feedstock for the production of biofuel. Genetic engineering and genome editing have tr...