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Morphological and biochemical characteristics of genetically transformed roots of Scutellaria andrachnoides
Genetically transformed roots (hairy roots) and callus tissue of skullcap (Scutellaria andrachnoides Vved.) were for the first time introduced in the in vitro culture. S. andrachnoides is the endemic plant of the...
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Article
Genetically transformed roots as a model system for studying physiological and biochemical processes in intact roots
The sequence of operations during plant genetic transformation using wild strains of the soil bacterium Agrobacterium rhizogenes and subsequent root production capable of long-term growth on the relatively simple...
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Article
Artificial seeds as a way to produce ecologically clean herbal remedies and to preserve endangered plant species
Influence of entrapment of the segments of isolated pRi T-DNA transformed roots of skullcap (Scutellaria baicalensis Georgi) in alginate gel capsules on the morphological, anatomical, physiological, and biochemic...
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Article
Artificial seed preparation as the efficient method for storage and production of healthy cultured roots of medicinal plants
The technique for the refinement of pRi T-DNA-transformed root cultivation by the root fragment encapsulation in the gel coat, i.e., so-called “artificial seed” (AS) production, was studied. AS were produced f...
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Article
Composition of essential oil in genetically transformed roots of Ruta graveolens
The composition of essential oil obtained by hydrodistillation from genetically transformed roots of common rue (Ruta graveolens L.) was analyzed. Using gas chromatography and complex gas chromatography-mass spec...
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Article
Isolation and identification of 4′,6-dimethoxy-7-hydroxyisoflavone from roots of Hedysarum theinum cultivated in vitro
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Article
Formation of phenolic compounds in the roots of Hedysarum theinum cultured in vitro
A steadily growing culture of genetically transformed roots of a valuable medicinal Altaic plant Hedysarum theinum Krasnob. was established using a Ri-plasmid (pRi) T-DNA of A4 wild strain of Agrobacterium rhizog...
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Article
HPLC Analysis of Alizarin and Purpurin Produced by Rubia tinctorum L. Hairy Root Cultures
We have determined the quantities of the anthraquinones alizarin and purpurin, with especial regard to their effective antigenotoxic activity, in genetically transformed hairy root cultures of Rubia tinctorum L. ...
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Article
GC and GC-MS Studies on the Essential Oil and Thiophenes from Tagetes patula L.
The production and the composition of the compounds (mono-, sesquiterpenes and acetylenic thiophenes) obtained by the steam distillation of Tagetes patula L. have been investigated. The volatile oil was produced ...
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Article
Flavones in genetically transformed Scutellaria baicalensis roots and induction of their synthesis by elicitation with methyl jasmonate
The composition and content of flavones were estimated in pRi T-DNA-transformed skullcap (Scutellaria baicalensis Georgi) roots obtained by the inoculation of axenically grown seedlings with a wild A4 strain of t...
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Article
HPLC Determination of Flavonoids in Hairy-Root Cultures of Scutellaria baicalensis Georgi
A simple and sensitive liquid chromatographic method has been established for determination of the main flavonoid components of Scutellaria baicalensis Georgi (Lamiaceae) roots and hairy-root cultures. Wogon, the...
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Article
Genetically Transformed Plant Roots as a Model for Studying Specific Metabolism and Symbiotic Contacts of the Root System
Genetic transformation of plants mediated by Ri plasmid ofAgrobacterium rhizogenes occupies a special place in plant cell engineering, since this technique based on a natural phenomenon allows cultivation of isol...
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Article
Flavonoid Production in Transformed Scutellaria baicalensis Roots and Ways of Its Regulation
A root culture of skullcap (Scutellaria baicalensisGeorgi) transformed with pRi T-DNA was initiated by the inoculation of sterile seedlings with Agrobacterium rhizogenes(wild-type strain A-4). The flavonoid conce...
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Are tissue cultures of Peganum harmala a useful model system for studying how to manipulate the formation of secondary metabolites?
This article reviews our present knowledge on the formation of tryptophan derived secondary metabolites in tissue cultures of Peganum harmala. With the presence of β-carboline alkaloids and serotonin, P. harmala ...
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Article
Separation of indole alkaloids fromR. serpentina andR. vomitoria by HPLC and TLC methods
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Article
Are tissue cultures of Peganum harmala a useful model system for studying how to manipulate the formation of secondary metabolites?
This article reviews our present knowledge on the formation of tryptophan derived secondary metabolites in tissue cultures of Peganum harmala. With the presence of β-carboline alkaloids and serotonin, P. harmala ...
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Article
Acridone alkaloids of callus tissue ofRuta graveolens
A strain of tissue of common rue of root origin (index of the strain R-4) has been obtained which produces up to 3% (of the dry weight) of acridone alkaloids. It has been shown by physicochemical methods that ...
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Article
Biosynthesis of rutacridone in tissue cultures of Ruta graveolens L.
The dihydrofuroacridone, rutacridone, is the main alkaloid in tissue cultures of Ruta graveolens L. strain R-19. The biosynthesis of this particular acridone alkaloid was investigated by using calluses and suspen...