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  1. Article

    Cas13d knockdown of lung protease Ctsl prevents and treats SARS-CoV-2 infection

    SARS-CoV-2 entry into cells requires specific host proteases; however, no successful in vivo applications of host protease inhibitors have yet been reported for treatment of SARS-CoV-2 pathogenesis. Here we de...

    Zhifen Cui, Cong Zeng, Furong Huang, Fuwen Yuan, **gyue Yan… in Nature Chemical Biology (2022)

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    Purification of Chinese Sacbrood Virus (CSBV), Gene Cloning and Prokaryotic Expression of its Structural Protein VP1

    The aim of this study was to purify the Chinese Sacbrood Virus Bei**g Miyun (BJMY-CSBV) from infected Apis cerana larvae, clone structural protein gene VP1 (named BJMY-CSBV-VP1), and investigate its biological i...

    Pengjie Wu, Huimin Yu, ** Xu, Jiangli Wu, Awraris Getachew… in Molecular Biotechnology (2018)

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    Isolation of a Novel Lipase Gene from Serratia liquefaciens S33 DB-1, Functional Expression in Pichia pastoris and its Properties

    A new lipase gene designated as SlLipA was isolated from Serratia liquefaciens S33 DB-1 by the genomic-walking method. The cloned gene contained an open reading frame (ORF) of 1,845 bp encoding 615 amino acids wi...

    Hongyan Yao, Shunwu Yu, Lida Zhang, Kai**g Zuo, Hua Ling… in Molecular Biotechnology (2008)

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    Rationalisation of metal binding to transferrin: Prediction of metal-protein stability constants

    Serum transferrin is an 80 kDa glycoprotein which transports Fe3+ and a variety of metal ions of diagnostic, therapeutic and toxic importance. Methods for the study of the differences between the two metal bindin...

    Dr Hongzhe Sun, Mark C. Cox, Hongyan Li… in Metal Sites in Proteins and Models (1997)