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  1. No Access

    Article

    Production of recombinant granulocyte colony-stimulating factor by knocking into the active immunoglobulin heavy chain gene locus in the hybridoma cell line

    The hybridoma cell line KM50 originally produces a monoclonal antibody at a concentration of ∼40 mg ml-1 in ascites. To investigate the possibility to apply this expression system to the production of useful prot...

    Yoshihisa Kuwana, Kikuko Funayama, Hiromasa Miyaji, Mamoru Hasegawa… in Cytotechnology (2001)

  2. No Access

    Article

    Microbial production of abscisic acid with Cercospora rosicola. 1. Stimulation of abscisic acid accumulation by plant extracts

    (+)-cis-trans-Abscisic acid, the natural isomer, accumulated in the culture medium of Cercospora rosicola grown on 2.4% potato dextrose broth with shaking; addition of narcissus extract stimulated the accumulatio...

    Takako Takayama, Hajime Yoshida, Kazumi Araki, Kiyoshi Nakayama in Biotechnology Letters (1983)

  3. No Access

    Article

    Microbial production of abscisic acid with Cercospora rosicola. II. Effect of pH control and medium composition

    Culture conditions for abscisic acid accumulation by Cercospora rosicola were tested. Additions of corn steep liquor and CaCO3 as pH controller to potato dextrose medium raised ABA accumulation to 300 ug/ml or mo...

    Takako Takamaya, Hajime Yoshida, Kazumi Araki, Kiyoshi Nakayama in Biotechnology Letters (1983)