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Article
Chromosome banding in Amphibia
The karyotype of the marsupial frog Gastrotheca riobambae is characterized by exceptionally highly differentiated XY♂/XX♀ sex chromosomes. The 18S and 28S ribosomal RNA genes were found only in the nucleolus orga...
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Article
Ribosomal gene amplification in multinucleate oocytes of the egg brooding hylid frog Flectonotus pygmaeus
The multinucleate oocytes of Flectonotus pygmaeus begin as cysts containing 2,000 or more meiotic nuclei. Each nucleus amplifies its ribosomal DNA early in oogenesis. The level of amplification is widely differen...
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Article
In situ hybridization of highly repetitive DNA to chromosomes of Triturus cristatus
Highly repetitive DNA of C0t 0–0.2 was purified from whole DNA of Triturus cristatus carnifex, labelled by nick translation, and in situ hybridized to RNA transcripts on the loops of lampbrush chromosomes and to ...
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Article
The arrangement and transcription of “middle repetitive” DNA sequences on lampbrush chromosomes of Triturus
Nick-translated 3H middle repetitive DNA (C0t 0.2–50) from Triturus cristatus carnifex was in situ hybridized to RNA transcripts on the loops of lampbrush chromosomes and to the denatured DNA in mitotic chromosom...
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Article
Chromosomes, DNA sequences, and evolution in salamanders of the genus Aneides
Chromosomes and DNA sequence homologies have been studied in salamanders of the genus Aneides. The species studied included A. ferreus, flavipunctatus, lugubris, hardii and aeneus. All species have 14 chromosomes...
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Article
In situ hybridization of “Nick-translated” 3H-ribosomal DNA to chromosomes from salamanders
A technique is described for preparation of 3H-labelled DNA by nick-translation employing deoxyribonuclease I and DNA polymerase I. The labelled DNA can be obtained in high yield with specific activities of 106 c...
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Article
Chromomere number and its genetic significance in lampbrush chromosomes
The chromosomes of three species of salamander belonging to the genus Plethodon have been studied with regard to the absolute length of the haploid set of lampbrush chromosomes and the number and distribution of ...
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Article
In situ hybridization of ribosomal DNA labelled with 125iodine to metaphase and lampbrush chromosomes from newts
Methods are described for in situ hybridization of ribosomal DNA from Xenopus laevis, labelled in vitro with 125iodine, to mitotic and lampbrush chromosomes from Triturus cristatus carnifex. The hybridization rea...
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Article
The nucleolar organizer of Plethodon cinereus cinereus (Green)
The 7th longest lampbrush bivalent in oocytes of Plethodon cinereus cinereus has a region of attached oocyte nucleoli near to the centromere on the shorter arm of each half bivalent. When squash preparations of P...
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Article
The nucleolar organizer of Plethodon cinereus cinereus (Green)
The lampbrush nucleolar organizers in P. c. cinereus are on the shorter arms of the 7th longest bivalent, near to the centromeres. The organizer consists of a non-chromomeric, Feulgen-negative section of the half...
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Article
Observations on centromeric heterochromatin and satellite DNA in salamanders of the genus Plethodon
DNA from Plethodon cinereus cinereus separates into two fractions on centrifugation to equilibrium in neutral CsCl. The smaller of these fractions has been described as a high-density satellite. It represents abo...
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Article
The arrangement of chromosomes in nuclei of sperm from plethodontid salamanders
Plethodontid salamanders have n = 13 or 14 large metacentric or sub-metacentric chromosomes. Sperm nuclei from Plethodon cinereus measure 72×1 μm. The nucleoprotein of spermatids is at first finely granular. In e...
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Article
Interlocking and knotting of ring nucleoli in amphibian oocytes
Various configurations of interlocking and knotting of ring nucleoli from amphibian oocytes have been identified in material taken from 2 females of Eurycea bislineata and one female of Plethodon cinereus. The si...
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Article
The chromosomal localization of a heavy satellite DNA in the testis of Plethodon c. cinereus
When DNA from blood or liver of Plethodon c. cinereus is centrifuged to equilibrium in cesium chloride it separates out into 2 components. The smaller or satellite component is relatively rich in G + C and is the...
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Article
A fresh look at meiosis and centromeric heterochromatin in the red-backed salamander, Plethodon cinereus cinereus (Green)
Male meiosis, with special regard to the centromeric heterochromatin and to centromeric structure, has been studied in the salamander, Plethodon cinereus cinereus. In this salamander, n = 14. Early meiotic propha...