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Article
A Tribute to Professor Shigeki Mizuno
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Article
Transcription on lampbrush chromosomes of a centromerically localized highly repeated DNA in pigeon (Columba) relates to sequence arrangement
A highly repetitive, centromerically localized DNA sequence (PR1) has been isolated from the genomic DNA of two species of pigeon (Columba livia andC. palumbus). PR1 is approximately 900 bp long. It includes a se...
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Article
The arrangement and transcription of telomere DNA sequences at the ends of lampbrush chromosomes of birds
The arrangement of loops and chromomeres at the ends of lampbrush chromosomes in four species of bird is described with reference to chromomeres, loops and transcription units. Unlike the situation described i...
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Article
Chromosome banding in Amphibia
The karyotype of the marsupial frog Gastrotheca riobambae is characterized by exceptionally highly differentiated XY♂/XX♀ sex chromosomes. The 18S and 28S ribosomal RNA genes were found only in the nucleolus orga...
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Chapter
The evolutionary consequence of major genomic changes in Amphibia
It seems appropriate to begin this discussion by examining the kinds of major genomic changes that have happened in the course of amphibian evolution, and to segregate those that may have been expected to cont...
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Chapter
Abstracts of Selected Posters
The analysis of chromosomes of mouse embryos permits detection of transmitted chromosome damage. The protocol involves the treatment of one parent (usually the male) with a clastogen, recovery of embryos and p...
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Article
Ribosomal gene amplification in multinucleate oocytes of the egg brooding hylid frog Flectonotus pygmaeus
The multinucleate oocytes of Flectonotus pygmaeus begin as cysts containing 2,000 or more meiotic nuclei. Each nucleus amplifies its ribosomal DNA early in oogenesis. The level of amplification is widely differen...
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Chapter
The Transcription of Satellite and Ribosomal DNA Sequences on Lampbrush Chromosomes of Crested Newts
Over the past few years we have asked specifically with regard to lampbrush chromosomes of crested newts, how certain classes of DNA sequences are arranged and expressed and how, if at all, their arrangement r...
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Chapter and Conference Paper
The Transcription of Satellite and Ribosomal DNA Sequences on Lampbrush Chromosomes of Crested Newts
Over the past few years we have asked specifically with regard to lampbrush chromosomes of crested newts, how certain classes of DNA sequences are arranged and expressed and how, if at all, their arrangement r...
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Article
In situ hybridization of highly repetitive DNA to chromosomes of Triturus cristatus
Highly repetitive DNA of C0t 0–0.2 was purified from whole DNA of Triturus cristatus carnifex, labelled by nick translation, and in situ hybridized to RNA transcripts on the loops of lampbrush chromosomes and to ...
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Article
The arrangement and transcription of “middle repetitive” DNA sequences on lampbrush chromosomes of Triturus
Nick-translated 3H middle repetitive DNA (C0t 0.2–50) from Triturus cristatus carnifex was in situ hybridized to RNA transcripts on the loops of lampbrush chromosomes and to the denatured DNA in mitotic chromosom...
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Article
Chromosomes, DNA sequences, and evolution in salamanders of the genus Aneides
Chromosomes and DNA sequence homologies have been studied in salamanders of the genus Aneides. The species studied included A. ferreus, flavipunctatus, lugubris, hardii and aeneus. All species have 14 chromosomes...
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Article
In situ hybridization of “Nick-translated” 3H-ribosomal DNA to chromosomes from salamanders
A technique is described for preparation of 3H-labelled DNA by nick-translation employing deoxyribonuclease I and DNA polymerase I. The labelled DNA can be obtained in high yield with specific activities of 106 c...
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Article
Chromomere number and its genetic significance in lampbrush chromosomes
The chromosomes of three species of salamander belonging to the genus Plethodon have been studied with regard to the absolute length of the haploid set of lampbrush chromosomes and the number and distribution of ...
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Article
In situ hybridization of ribosomal DNA labelled with 125iodine to metaphase and lampbrush chromosomes from newts
Methods are described for in situ hybridization of ribosomal DNA from Xenopus laevis, labelled in vitro with 125iodine, to mitotic and lampbrush chromosomes from Triturus cristatus carnifex. The hybridization rea...
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Article
The nucleolar organizer of Plethodon cinereus cinereus (Green)
The 7th longest lampbrush bivalent in oocytes of Plethodon cinereus cinereus has a region of attached oocyte nucleoli near to the centromere on the shorter arm of each half bivalent. When squash preparations of P...
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Article
The nucleolar organizer of Plethodon cinereus cinereus (Green)
The lampbrush nucleolar organizers in P. c. cinereus are on the shorter arms of the 7th longest bivalent, near to the centromeres. The organizer consists of a non-chromomeric, Feulgen-negative section of the half...
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Article
Observations on centromeric heterochromatin and satellite DNA in salamanders of the genus Plethodon
DNA from Plethodon cinereus cinereus separates into two fractions on centrifugation to equilibrium in neutral CsCl. The smaller of these fractions has been described as a high-density satellite. It represents abo...
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Article
The arrangement of chromosomes in nuclei of sperm from plethodontid salamanders
Plethodontid salamanders have n = 13 or 14 large metacentric or sub-metacentric chromosomes. Sperm nuclei from Plethodon cinereus measure 72×1 μm. The nucleoprotein of spermatids is at first finely granular. In e...
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Article
Interlocking and knotting of ring nucleoli in amphibian oocytes
Various configurations of interlocking and knotting of ring nucleoli from amphibian oocytes have been identified in material taken from 2 females of Eurycea bislineata and one female of Plethodon cinereus. The si...