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Article
Human peripheral blood monuclear cells transfected with messenger RNA stimulate antigen-specific cytotoxic T-lymphocytes in vitro
The efficiency of test vaccines needs to be evaluated by quantification of the triggered cellular immune response. Usually, for these assays, autologous target cells expressing the vaccine antigen are required...
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Article
Rituximab mediates in vitro antileukemic activity in pediatric patients after allogeneic transplantation
Relapse is a major problem after allogeneic transplantation in children with acute B-lineage lymphoblastic leukemias (ALL) and lymphomas and additional therapeutic strategies are needed to increase graft versus l...
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Article
Polarization of immunity induced by direct injection of naked sequence-stabilized mRNA vaccines
In the context of develo** a safe genetic vaccination strategy we tested and studied globin-stabilized mRNA-based vaccination in mice. This vaccination strategy has the advantages of genetic vaccination (ea...
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Article
CD4+ T cell-mediated immunity against prion proteins
The prion protein (PrPC) is essential for susceptibility to transmissible spongiform encephalopathies. A specific conformer of this protein (PrPSc) is, according to the 'protein only' hypothesis, the principal o...
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Article
Degradation of desferrioxamines by Azospirillum irakense: Assignment of metabolites by HPLC/electrospray mass spectrometry
Based on a recent finding that an Azospirillum isolate ASP-1 possessing high 16S rDNA similarity to Azospirillum irakense was able to degrade desferrioxamine type siderophores (Winkelmann et al. BioMetals 9, 7...
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Article
pCOR: a new design of plasmid vectors for nonviral gene therapy
A totally redesigned host/vector system with improved properties in terms of safety has been developed. The pCOR plasmids are narrow-host range plasmid vectors for nonviral gene therapy. These plasmids contain...
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Chapter
Peptide Libraries in Cellular Immune Recognition
Protective immunity against microbial pathogens is connected with the ability to discriminate between self and non-self. Vertebrates have evolved an adaptive immune system in which specialized white blood cell...
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Article
Two types of modified cardiac Na+ channels after cytosolic interventions at the α-subunit capable of removing Na+ inactivation
Failure of inactivation is the typical response of voltage-gated Na+ channels to the cytosolic presence of proteolytic enzymes, protein reagents such as N-bromoacetamide (NBA) or iodate, and antibodies directed ...
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Article
Effects of polycations on ion channels formed by neutral and negatively charged alamethicins
The effects of the peptide polycations salmon protamine (M r = 4332,z = + 21) and poly-l-lysine (M r ≅ 100,00,z ≅ + 775) on ion channels formed by synthetic alamethicin Alm-F30 (one negative charge), natural Alm-...
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Article
Purification and chemical characterization of staphyloferrin B, a hydrophilic siderophore from staphylococci
This paper describes the chemical characterization of staphyloferrin B, a new complexone type siderophore isolated from low iron cultures of Staphylococcus hyicus DSM 20459. Purification of the very hydrophilic m...
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Article
Microtubules and cell sha** in the mesophyll ofNigella damascena L.
Cell sha** in the mesophyll ofNigella damascena was investigated with the aim of determining the origin of the arm-like protrusions, which are characteristic of, e.g., arm-palisade cells. It was found that hoop...
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Article
Cell sha** and microtubules in develo** mesophyll of wheat (Triticum aestivum L.)
Differentiated mesophyll cells ofTriticum aestivum (cv. Star) exhibit a lobed outline resembling tube-shaped balloons with almost regularly spaced constrictions. It was shown that these constrictions are probably...
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Article
Molecular shape and dipole moment of alamethicin-like synthetic peptides
The peptides Boc-(l-Ala-Aib-l-Ala-Aib-l-Ala)n-OMe, with n=2 (P10) and n=4 (P20), have been synthesized as purely hydrophobic models of the antibiotic alamethicin, which is known to be a voltage-dependent pore for...
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Article
Alamethicin pore formation: Voltage-dependent flip-flop of α-helix dipoles
The voltage-dependency of alamethicin pore formation is explained by a flip-flop gating mechanism of single alamethicin molecules. The energetically preferred aggregate structure is changed from antiparallel t...
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Article
Biological activity of the antitumor protein neocarzinostatin coupled to monoclonal antibody by N-succinimidyl 3-(2-pyridildithio) propionate (SPDP)
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Article
Nosiheptide, a sulfur-containing peptide antibiotic isolated fromStreptomyces actuosus 40037
Nosiheptide (9671 R.P.) isolated fromStreptomyces actuosus 40037 (NRRL 2954) is a sulfur-containing polypeptidic antibiotic, quite different from all the other members of this family. Very active in vitro against...