25 Result(s)
within
G. Danscher
English
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Article
Autometallographic tracing of Hg–S quantum dots in frogs exposed to inorganic mercury
The histochemical distribution of mercury in the kidneys and gut of frogs (Rana ridibunda) exposed to inorganic mercury was analyzed with autometallography (AMG). It was found that most mercury in the kidneys acc...
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Immersion autometallographic tracing of zinc ions in Alzheimer beta-amyloid plaques
An easy to perform autometallographic technique (AMG) for capturing zinc ions in Alzheimer plaques is presented. The possibility of visualizing loosely bound or free zinc ions in tissue by immersion autometall...
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Article
The effect of selenium on the localization of autometallographic mercury in dorsal root ganglia of rats
The autometallographic technique was used to demonstrate the localization of mercury in dorsal root ganglia of adult Wistar rats. The animals were either exposed to mercury vapour, 100 μg Hg m−3, 6 h day−1, 5 day...
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Article
The autometallographic zinc-sulphide method. A new approach involvingin vivo creation of nanometer-sized zinc sulphide crystal lattices in zinc-enriched synaptic and secretory vesicles
A new version of Timm's sulphide silver method involvingin vivo binding of zinc ions in zinc enriched terminals is presented. By injecting sodium sulphide into the vena cava of deeply anaesthetized animals, it is...
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Autometallographic silver-enhancement of colloidal gold particles used to label phagocytic cells
The present paper demonstrates that colloidal gold silver-enhanced by autometallography (AMG) can be used to label phagocytic cells for light microscopic detection. Cultured macrophages were exposed to 0.5 μl ...
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Article
Densitometric analysis of the local bleaching of the Neo-Timm staining pattern following intrahippocampal injection of diethyldithiocarbamate
Treatment with certain metal chelating agents causes a time-dependent bleaching of the Neo-Timm staining pattern of zinc visualized in synaptic vesicles. In the present study, the extent and time course of the...
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Article
Autometallography used as a histochemical indicator of lysosome function in cultured cells
The present paper suggests the autometallographic demonstration of intralysosomal silver as a sensitive tool for the detection of subtile toxic effects in cell cultures, the method is suggested to primarily de...
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Article
Evidence from dithizone and selenium zinc histochemistry that perivascular mossy fiber boutons stain preferentially “in vivo”
This paper describes a perivascular staining pattern that is obtained when dithizone or sodium selenite are used to label zinc intravitally. Our observations indicate that the perivascular staining is a result...
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Article
Quantification of vesicular zinc in the rat brain
By means of the Neo-Timm method it has recently been shown that zinc is present in a fraction of the round clear synaptic vesicles of certain boutons located primarily in telencephalic structures (Pérez-Clause...
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Article
Can aluminium be visualized in CNS by silver amplification?
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Article
Autometallography: Tissue metals demonstrated by a silver enhancement kit
In biological tissue, minute accumulations of gold, silver, mercury and zinc can be visualized by a technique whereby metallic silver is precipitated on tiny accumulations of the two noble metals, or on seleni...
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Autometallographic demonstration of gold in human fetal liver and placenta
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Article
Differentiation of histochemically visualized mercury and silver
Accumulations of silver and mercury can be visualized in tissue sections by a technique called autometallography or physical development. In order to make a histological differentiation between mercury and sil...
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Article
The dithizone, Timm's sulphide silver and the selenium methods demonstrate a chelatable pool of zinc in CNS
From rats intravitally treated with dithizone (diphenyl-thiocarbazone) brains and spinal cords were removed and freeze-dried. The dithizonates present in the CNS tissue were extracted with carbon tetrachloride...
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Article
Autometallography
The autometallographic procedure represents a new technique that can substitute for the normal methods of physical development (PD). The physical developer (a solution of reducing substance, silver salt and pr...
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Article
Energy dispersive X-ray analysis of tissue gold after silver amplification by physical development
Rats were treated intraperitoneally with the gold-containing compounds sodium aurothiomalate (Myocrisin), sodium aurothiosulfate (Sanocrysin), and aurothioglucose. Using stem energy dispersive X-ray analysis, ...
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Article
Neuronal accumulation of silver in brains of progeny from argyric rats
Using a photochemical method silver was demonstrated in the brains of 1-, 14-, and 45-day-old rats which had been exposed to silver on gestational days 18 and 19. In the brain tissue of new-born rats, silver w...
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Localization of exogenous silver in brain and spinal cord of silver exposed rats
Exogenous silver in brain and spinal cord sections from rats treated with Protargol, silver lactate or silver nitrate was visualized by physical development. The silver penetrated the blood-brain barrier and a...
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Article
Exogenous selenium in the brain
Transcardial perfusion or intraperitoneal injections with sodium selenite result in the creation of selenium bonds that can be visualized by physical development. The present paper describes how these catalyti...
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Article
Light and electron microscopic localization of silver in biological tissue
A method is described that visualizes trace amounts of silver in frozen, paraffin and epon sections from biological tissue. After exposure to light, which ensures reduction of silver ions that are not bound to...
