16 Result(s)
within
G. Danscher
Biomedicine
English
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Article
Immersion autometallographic tracing of zinc ions in Alzheimer beta-amyloid plaques
An easy to perform autometallographic technique (AMG) for capturing zinc ions in Alzheimer plaques is presented. The possibility of visualizing loosely bound or free zinc ions in tissue by immersion autometall...
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Article
Autometallographic silver-enhancement of colloidal gold particles used to label phagocytic cells
The present paper demonstrates that colloidal gold silver-enhanced by autometallography (AMG) can be used to label phagocytic cells for light microscopic detection. Cultured macrophages were exposed to 0.5 μl ...
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Article
Autometallography used as a histochemical indicator of lysosome function in cultured cells
The present paper suggests the autometallographic demonstration of intralysosomal silver as a sensitive tool for the detection of subtile toxic effects in cell cultures, the method is suggested to primarily de...
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Article
Evidence from dithizone and selenium zinc histochemistry that perivascular mossy fiber boutons stain preferentially “in vivo”
This paper describes a perivascular staining pattern that is obtained when dithizone or sodium selenite are used to label zinc intravitally. Our observations indicate that the perivascular staining is a result...
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Article
Quantification of vesicular zinc in the rat brain
By means of the Neo-Timm method it has recently been shown that zinc is present in a fraction of the round clear synaptic vesicles of certain boutons located primarily in telencephalic structures (Pérez-Clause...
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Article
Autometallography: Tissue metals demonstrated by a silver enhancement kit
In biological tissue, minute accumulations of gold, silver, mercury and zinc can be visualized by a technique whereby metallic silver is precipitated on tiny accumulations of the two noble metals, or on seleni...
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Article
The dithizone, Timm's sulphide silver and the selenium methods demonstrate a chelatable pool of zinc in CNS
From rats intravitally treated with dithizone (diphenyl-thiocarbazone) brains and spinal cords were removed and freeze-dried. The dithizonates present in the CNS tissue were extracted with carbon tetrachloride...
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Article
Autometallography
The autometallographic procedure represents a new technique that can substitute for the normal methods of physical development (PD). The physical developer (a solution of reducing substance, silver salt and pr...
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Article
Energy dispersive X-ray analysis of tissue gold after silver amplification by physical development
Rats were treated intraperitoneally with the gold-containing compounds sodium aurothiomalate (Myocrisin), sodium aurothiosulfate (Sanocrysin), and aurothioglucose. Using stem energy dispersive X-ray analysis, ...
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Article
Exogenous selenium in the brain
Transcardial perfusion or intraperitoneal injections with sodium selenite result in the creation of selenium bonds that can be visualized by physical development. The present paper describes how these catalyti...
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Article
Light and electron microscopic localization of silver in biological tissue
A method is described that visualizes trace amounts of silver in frozen, paraffin and epon sections from biological tissue. After exposure to light, which ensures reduction of silver ions that are not bound to...
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Article
Localization of gold in biological tissue
A detailed description is given of a method by which gold can be visualized in frozen, paraffin and Epon sections. Histological sections from animals treated with gold compounds are exposed to UV-light from 30...
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Article
Histochemical demonstration of heavy metals
The three steps of the sulphide silver method have been examined: 1) Transformation of metals to metal sulphides; 2) Fixation and embedding or freezing of the tissue for sectioning; and 3) Deposition of metall...
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Article
Electron microscopic demonstration of metals in rat mast cells
This paper describes a modification of a cytochemical method for the demonstration of heavy metals. The well localized precipitate in the mast cell granules, which is also present in granules that have been se...
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Article
Multi-element analysis of the rat hippocampus by proton induced X-ray emission spectroscopy (phosphorus, sulphur, chlorine, potassium, calcium, iron, zinc, copper, lead, bromine, and rubidium)
A technique for multi-element analysis of brain tissue by proton induced X-ray emission spectroscopy (PIXE) is described and data from analysis of fixed and unfixed samples from rat hippocampus, neocortex, amy...
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Article
Histochemical demonstration of mercury induced changes in rat neurons
A histochemical method modified for ultrastructural studies of mercury induced changes is described. Rat neurons from areas known to be influenced by mercury are used as examples. The histochemical reaction, s...
